Aims: The objective of this study was to establish the validity of biochemical markers in osteoporosis evaluation by comparing the biochemical marker indices with that of T-scores values, considered to be the gold standard of osteoporosis assessment. Methods: Sixty osteoporotic postmenopausal Indian women were recruited in the study on the basis of DEXA T-scores. The biochemical markers estimated were Crosslaps (bone degradation marker), Osteocalcin (bone formation marker), and intact PTH and vitamin D (associated markers). Serum Osteocalcin and 25-OH Vitamin D were estimated by Radioimmunoassay. Intact PTH in the plasma was estimated by Chemiluminescence. Crosslaps was estimated in the second morning void by Enzyme Linked Immuno Sorbent Assay and expressed in terms of mmol creatinine. Results: The results of the present study showed that Crosslaps, the resorption marker was signiþcantly higher (signiþcant at P< 0.5) in osteoporotic patients as compared to that of the controls (n=27). The other three markers were comparable in both the groups and were within the normal range. The most significant þnding of this study was that the resorption marker and DEXA results showed a signiþcant correlation (R=0.82). Conclusions: Of the four biochemical markers assayed, Crosslaps turned out to be the best predictor of the metabolic turnover changes and the values signiþcantly correlated with that of the DEXA scores.