Advertisement for orthosearch.org.uk
Orthopaedic Proceedings Logo

Receive monthly Table of Contents alerts from Orthopaedic Proceedings

Comprehensive article alerts can be set up and managed through your account settings

View my account settings

Visit Orthopaedic Proceedings at:

Loading...

Loading...

Full Access

O1416 A NEW BIOREACTOR FOR MECHANICAL LOADING OF TISSUE AND SCAFFOLDS IN LONGTERM PERFUSION CULTURE



Abstract

Introduction: Due to a lack of techniques there is only some data of testing mechanical influence on chondroc-tyes grown in 3-D tissue-culture over several months. The authors developed therfore a new perfusion-chamber to study these mechanical factors in in-vitro tissue culture. Methods: A pneumatic computer-controlled bioreactor which allows the application of pressure between 0,1 – 2 MPa in frequencies less than 1 Hz over a period of several months was developed. The reactor is made of stainless steel and closed to the surrounding atmosphere with a steril, flexible and pressure resistant layer of film. 8 scaffolds with a maximal diameter of 10 mm and a maximal height of 15 mm can be studied independently in respect to pressure parameters. A continous flow of medium allows an excellent nutrition, temperature is controlled by a closed heat unit. In a first system three bioreactors are used simultaneousely. Results: In first cultures of tissues over a period of three months sterility was well contained without any mechanical problems. With a high degree of automation a nearly uncontrolled running was possible.

Theses abstracts were prepared by Professor Dr. Frantz Langlais. Correspondence should be addressed to him at EFORT Central Office, Freihofstrasse 22, CH-8700 Küsnacht, Switzerland.