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MANIPULATED OSTEOGENIC BONE ALLOGRAFTS: A SOUTH AFRICAN TISSUE BANKING PERSPECTIVE



Abstract

We present data showing predictable bone-inducing capacity of two types of tissue-bank manipulated bone allograft, which is used increasingly as biological allograft.

The first type is an in-vitro tested demineralised bone matrix (DBM) composition of human long bone, which contains lyophilised cross-linked collagenous biomaterial as a delivery system. The composition is in the form of an allograft powder that is stable at room temperature and readily rehydratable into a putty-like consistency. Its superior co-adhesive properties enable it to remain at the implant site, resisting irrigation and displacement due to bleeding. We discuss a number of cases in which successful fusion of recalcitrant long bone fractures in humans followed implantation of the DBM-collagenous biomaterial composite.

We also present data on a second type of manipulated human allograft, a reconstituted DBM with increased levels of osteogenic fractions derived from human cortical bone. The osteogenic fraction is rich in bone morphogenetic protein (BMP) and capable of inducing alkaline phosphatase activity in in vitro cultures of rat myoblast cells. Alkaline phosphatase is a well-known specific marker for bone forming activity. Thirty-four patients with persistent non-union (including partial or complete segmental defects) were treated with the BMP-collagen composite. All patients had previously been treated by internal or external fixation, cast immobilisation, and/or allogenic or autogenic bone grafting. Preoperative symptoms averaged 26 months (1 to 228). At surgery the hydrated implant was injected at the site of the defect, which was then stabilised by internal or external fixation. Early radiographs showed moderate bridging in all patients, indicating that h-BMP composite implants may be effective treatment of difficult nonunions.

Secretary: Dr H.J.S. Colyn, Editor: Professor M.B.E. Sweet. Correspondence should be addressed to SAOA, Box 47363, Parklands, Johannesburg, 2121, South Africa.