In vivo kinematic analyses of total hip arthroplasty (THA) have determined femoral head separation from the medial aspect of the acetabular component can occur. Various bearing materials are currently used in THA today. The objective of this study was to determine if differences in the incidence and magnitude of femoral head separation exist among various bearing surfaces for THA during different weight-bearing activities. 205 clinically successful subjects implanted with either metal-on-metal (MOM), metalon-polyethylene (MOP), ceramic-on-ceramic (COC) or ceramic-on-polyethylene (COP) materials were analyzed using video-fluoroscopy. Each patient performed either gait on a treadmill or an abduction-adduction activity. The fluoroscopic information was then analyzed using a computer aided 3D model fitting technique to determine the incidence and magnitude of hip separation. Additional variables analyzed included femoral head diameter, follow-up duration, and type of surgical approach utilized. Less separation was noted with increasing femoral head diameter during abductionadduction. Increased separation was observed during gait as follow-up duration increased. Hip separation was greater during gait when a posterolateral surgical approach was used but was greater in abduction-adduction if a antero-lateral approach was selected. The incidence and magnitude of hip separation during gait was least in subjects with COC THA and least with COC and MOM THA when analyzed during abduction-adduction. It’s been proposed that THA patients are subject to femoral head separation due to alterations in the soft tissue supporting structures during THA that affect constraint of the joint. The current analysis demonstrates lower magnitudes and incidence of THA separation occur when hard-on-hard bearing surfaces are selected and can vary based on femoral head diameter, follow-up duration, and surgical approach used. Potential detrimental effects resulting from THA separation include premature polyethylene wear, component loosening (secondary to impulse loading conditions) and hip instability.