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Orthopaedic Proceedings
Vol. 99-B, Issue SUPP_8 | Pages 69 - 69
1 Apr 2017
Barnouin L Günzel E
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Background

Based on decellularisation and cleaning processes of trabecular bone and fibrocartilage, an osteochondral allograft has been developed.

Material

The chemical process, established thanks to bone and fibrocartilage data, included an efficient viroinactivation step. The raw material was a tibial plateau collected during knee arthroplasty, cut in cylinders strictly selected (>2mm cartilage height and total height between 10 and 16mm). The grafts were freeze-dried and gamma sterilised.


Orthopaedic Proceedings
Vol. 99-B, Issue SUPP_8 | Pages 70 - 70
1 Apr 2017
Günzel E Barnouin L Delépine P Le Pape F
Full Access

Background

Meniscal tears are among the most common knee injuries. To preserve as much as possible the joint, partial and total meniscal replacements are necessary. To combine the biocompatibility and mechanical resistance of meniscus allograft with the disponibility of synthetic substitutes, an acellular, viroinactivated and sterile scaffold with well-preserved structure has been developed based on PHOENIX process.

Methods

Human menisci were collected from living donors undergoing total knee arthroplasty. They underwent chemical treatments, freeze-drying and gamma irradiation. Decellularisation of menisci and preservation of the matrix structure were explored by histological studies. Meniscal scaffold ultrastructure was analysed by scanning electron microscopy. Biomechanical studies were also conducted. Scaffold viroinactivation was investigated by viral clearance studies. Finally, the allografts were cultured for 4 weeks with Mesenchymal Stem Cells (CSM); cells viability and proliferation were assessed histologically and by confocal microscopy following stainings.