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Orthopaedic Proceedings
Vol. 100-B, Issue SUPP_17 | Pages 90 - 90
1 Dec 2018
El Sayed F Roux A Bauer T Nich C Sapriel G Dinh A Gaillard J Rottman M
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Aim

Cutibacterium acnes, a skin commensal, is responsible for 5–10% of prosthetic joint infections (PJI). All current microbiological definitions of PJI require two or more identical commensal isolates to be recovered from the same procedure to diagnose PJI and rule out contamination. Unlike coagulase negative staphylococci, C.acnes shows a highly stereotypical susceptibility profile making impossible to phenotypically assess the clonal relationship of isolates. In order to determine the clonal relationship of multiple C.acnes isolates recovered from arthroplasty revisions, we analyzed by multi-locus sequence typing (MLST) C.acnes isolates grown from orthopedic device-related infections (ODRI) in a reference center for bone and joint infection.

Methods

Laboratory records from January 2009 to January 2014 were searched for monomicrobial C.acnes ODRI with growth of C. acnes in at least 2 intraoperative and/or preoperative samples. Clinical, biological and demographic information was collected from hospital charts. All corresponding isolates biobanked in cryovials (−80°C) were subcultured on anaerobic blood agar, and identification confirmed by MALDI-TOF-MS. C.acnes isolates were typed using the MLST scheme described by Lomholt et al. Plasmatic pre-operative C-reactive protein (CRP) levels were determined using DimensionEXL (Siemens). A threshold of 10 mg/L was used to determine serologically positive ODRIs from negatives.


Orthopaedic Proceedings
Vol. 99-B, Issue SUPP_22 | Pages 34 - 34
1 Dec 2017
El Sayed F Gruber A Sapriel G Fawal N Bauer T Heym B Dupont C Hello SL Gaillard J Rottman M
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Aim

According to Tsukuyama classification, late acute hematogenous prosthesis joint infections (PJI) should be treated with debridement and implant retention (DAIR). We report here a recurrent Salmonella Dublin hip prosthesis infection. Through this case, we show how a recurrence of chronic PJI may have an acute clinical presentation leading to an inadequate surgical treatment.

Method

Case report. On May 2011, a 74-year-old woman with bilateral hip prostheses (implanted in 1998 (right) and 2001 (left)), was admitted to intensive care for sepsis and pain of her left hip. Blood cultures and a joint aspiration of the left hip yielded pure cultures of S.Dublin. The patient had a recent history of febrile diarrhea after consuming dubious meat. The patient underwent DAIR followed by a six-week antibiotherapy. Three years later, she presented to the emergency room for an acute onset febrile PJI of the right hip. The patient underwent DAIR of the right hip. Blood cultures, joint aspiration fluid, and all intraoperative periprosthetic tissue samples yielded S.Dublin. Colonoscopy and abdomen ultrasound were negative. The patient received two weeks of intravenous combined antibiotherapy followed by oral antibiotics for further 10 weeks. Six weeks post operatively, the surgical wound was healed and the patient walked normally. One year later, the patient was referred by her primary care practitioner for night fevers without local signs or dysfunction of her prostheses. Radioleucoscintigraphy showed right hip inflammation. Bilateral hip biopsies were nevertheless performed, yet S. Dublin was recovered solely from the right hip biopsy. A one-stage exchange of the right hip was performed. All intraoperative periprosthetic tissue samples yielded S.Dublin. A six-week-combined antibiotherapy was undertaken. One year later, the patient appeared free of infection and walked normally.


Orthopaedic Proceedings
Vol. 99-B, Issue SUPP_22 | Pages 77 - 77
1 Dec 2017
El Sayed F Roux A Rabès J Mazancourt P Bauer T Gaillard J Rottman M
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Aim

Propionibacterium acnes is a skin commensal colonizing the deeper structures of the pilous bulb. It is responsible for 5–10% of lower limb prosthetic joint infections (PJI) but accounts for as many as 50% of shoulder arthroplasty infections. P. acnes PJIs characteristically feature limited systemic inflammation, limited polymorphonuclear infiltration and clinical signs compatible with aseptic loosening. All current microbiological definitions of PJI require two or more identical commensal isolates to be recovered from the same procedure to diagnose PJI to increase specificity and rule out contamination. Whereas the antimicrobial susceptibility patterns of coagulase negative staphylococci are highly polymorphic and commonly allow the ready distinction of unrelated strains, P. acnes shows a highly stereotypical susceptibility profile and it is impossible to phenotypically assess the clonal relationship of isolates. In order to determine the clonal relationship of multiple P. acnes isolates recovered from arthroplasty revisions, we analyzed by multi-locus sequence typing (MLST) P. acnes isolates grown from PJI in a reference center for bone and joint infection.

Method

We retrospectively selected all cases of microbiologically documented monomicrobial PJI caused by P. acnes diagnosed in our center from January 2009 to January 2014. Microorganisms were identified by MALDI-TOF mass spectrometry (Bruker Daltonics). All corresponding P.acnes isolates biobanked in cryovials frozen at −80°C were subcultured on anaerobic blood agar, DNA extracted by freeze-thawing and bead-milling, and typed according to the 9 gene MLST scheme proposed by Lomholt HB. and al.


Orthopaedic Proceedings
Vol. 97-B, Issue SUPP_16 | Pages 75 - 75
1 Dec 2015
Marmor S Desplaces N Bauer T Heym B Sol O Rogé J Mahé F Desire L Ghout I Ropers J Gaillard J Rottman M
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The diagnosis of prosthetic joint infections (PJI) represents a critical challenge for orthopedic surgeons and infectious disease specialists. The diagnosis of PJI is often delayed because non-invasive assays lack sensitivity and specificity. A novel multiplex immunoassay detecting antibodies against Staphylococci, Propionibacteria and Streptococcus agalactiae was developed and its performance evaluated in a prospective, multicenter, non-interventional study.

The Luminex-based assay measures serum IgG against a proprietary panel of recombinant purified antigens from Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus lugdunensis, Streptococcus agalactiae and Propionibacterium acnes. Patients undergoing revision arthroplasty were included over a 2-year period (from 2012 up to 2014) in two French reference centers in compliance with IRB and French regulations. PJI cases were defined microbiologically (≥2 intraoperative samples yielding the same microorganism) for confrontation of microbiological and immunoassay data.

455 patients were eligible for study analyses. 149 patients (32.7%) were found to be infected. Among the most frequent infecting species recovered were S. aureus (30%), S. epidermidis (26%), P. acnes (9%), S. lugdunensis (6%), and S. agalactiae (4%). The sensitivity and specificity values of the test were, respectively, 75.9% (63/83) and 82.2% (180/219) for staphylococci (S. aureus, S. epidermidis, S. lugdunensis), 38.5% (5/13) and 81.9% (190/232) for P. acnes, and 66.7% (4/6) and 92.4% (208/225) for S. agalactiae. Interestingly, all cases (9/9) involving S. lugdunensis were detected by the test and the sensitivity for S. epidermidis reached 79.4% in patients more than three months after joint replacement. In a similar fashion, 89.5% (17/19) in the subpopulation with elevated inflammatory markers (ESR>30 and CRP>10).

The assay correctly identified 67% of the microbiologically positive patients that were negative by ESR or CRP screening.

This novel multiplex serological test allows the rapid and non-invasive diagnosis of the most frequent PJI pathogens, showing a good correlation with microbiological culture. and appears to be a new promising tool in the management of PJI, adding sensitivity to the current serological assays and enhancing the management of patients with pauci-inflammatory PJI.


Orthopaedic Proceedings
Vol. 87-B, Issue SUPP_III | Pages 252 - 252
1 Sep 2005
Rottman M Martin J Gaudin P Lortat-Jacob A Gaillard J Piriou P
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Introduction: The emergence of multidrug resistant Gram negative bacilli susceptible to hardly any beta lactam compound has led to infections close to a therapeutic dead end. In such circumstances, Imipenem-cilastatin (I-C) is often the only remaining therapeutic option. We report our experience with the prolonged administration of high-doses of I-C in the treatment of osteoarticular infections with bacteria resistant to other beta-lactam agents (or 4l generation cephalosporins in 14 cases).

Materials and methods: Our retrospective study over 7 years included 29 patients with septic arthritis (n=3) continuous osteitis (n=6), septic non-union(n=12) and prosthetic joint infections (n=8). Treatment included an extensive surgical debridement and post-operative combination antibiotherapy with intravenous I-C and aminoside (54%) and/or fluoroquinolones (46%) and/or fosfomycin (29%). Associated microorganisms requiring yet additional antimicrobial agents were associated in 17 (59%) cases. I-C was maintained for an average of 46 days (extremes 21–90), at an average dose of 3,8g/day (extremes 2–6). The bacteria warranting I-C were cephalosporinase hyperproducing Enterobacter cloacae (38%), extended spectrum beta-lactamases producing enterobacteria (31%), Pseudomonas aeruginosa (21%) and/or Acinetobacter baumanii (21%).

Results: Early outcome was favorable in 24 patients (82%). Two patients relapsed with the bacteria requiring I-C. Three failed to negate succion fluid cultures : one was discharged with no change in his condition, one agreed to a leg amputation and the third died of candidemic septic shock in SICU with drainage fluid still bactériologie ally positive. Repeated secondary colonization and systemic infection with yeasts led to a monitoring of yeast load. Per os amphotencin B and immediate treatment of urinary colonization prevented further systemic dissemination of candical infections. No other tolerance incidents were noted. Acquired resistance occurred only once in a P. aeruginosa isolate while Imipenem-cilastatin was chosen to cover an ESBL producing Escherichia coli. Secondary treatment with ceftazidime was then successful in eradicating P. aeruginosa.

Conclusion: I-C has been widely used for the treatment of mixed flora infections as a wide spectrum antibiotic.

We report good tolerance of high posology long term administration in documented osteoarticular indications if yeast colonization is properly monitored, and eradication rates are comparable to those reported in infections with susceptible bacteria.


Orthopaedic Proceedings
Vol. 84-B, Issue SUPP_I | Pages - 63
1 Mar 2002
Gleizes Y Bernard L Pron B Signoret F Feron J Gaillard J
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Purpose: The purpose of this study was to determine the usefulness of systematic bacteriological culture of drainage fluid after aseptic orthopaedic surgery in identifying infection early. There is much controversy over this point in the literature. In addition, the public health cost (approximately 30 euros per culture) must be considered in terms of effectivenes.

Material and methods: A prospective study was conducted in a single orthopaedic surgery department over a one-year period (1999) including all patients undergoing class I surgery (aseptic orthopaedic and traumatologic surgery). The population included 843 patients (52% men, 48% women, mean age 49 years, age range 15–98 years) who underwent 880 aseptic orthopaedic surgery procedures (osteosynthesis 60%, arthroplasty 30%, others 10%). One or several bacteriological cultures on early drainage fluid were performed (n=2434). The results of these cultures were analysed to determine their contribution to early detection of infection and rapid institution of adapted treatment (medical treatment with antibiotics or medical and surgical (revision) treatment).

Results: The bacteriological cultures were negative in 830 patients (98.5%) and positive in 13 (1.5%). A deep infection developed in 21 patients including 3 patients who had a positive drainage fluid culture and 18 who had a negative culture. In addition, ten patients had false positive cultures subsequent to extraneous contamination. The sensitivity, specificity and positive and negative predictive values were 14%, 98%, 23% and 98% respectively.

Discussion, conclusion: Drainage is a common procedure after orthopaedic surgery. The objective is to limit the risk of haematoma formation, but paradoxically with an increased risk of infection by retrograde contamination. The observed sensitivity and predictive values of drainage fluid cultures would suggest this is not a reliable method for detecting infection early, especially since the presence of a drain increases the risk of infection. In the final analysis, we do not recommend systematic culture of drainage fluid after aseptic orthopaedic surgery.