Poor tendon repair is an unsolved issue in clinical practice, due to complex tendon structure. Tendon stem/progenitor cells (TSPCs) play key roles in homeostasis, regeneration, and inflammation regulation in acute tendon injuries, and rely on TGF-β signaling for recruitment into degenerative tendons. In this study, we aimed to develop an in vitro model for tenogenesis adopting a dynamic culture of a fibrin 3D scaffold, bioengineered with human TSPCs collected from both healthy and tendinopathic surgery explants (Review Board prot./SCCE n.151, 29 October 2020). 3D culture was maintained for 21 days under perfusion provided by a custom-made bioreactor, in a medium supplemented with hTGF-β1 at 20 ng/mL. The data collected suggested that the 3D in vitro model well supported survival of both pathological and healthy cells, and that hTGF-β signaling, coupled to a dynamic environment, promoted differentiation events. However, pathological hTSPCs showed a different expression pattern of tendon-related genes throughout the culture and an impaired balance of pro-inflammatory and anti-inflammatory cytokines, compared to healthy hTSPCs, as indicated by qRT-PCT and immunofluorescence analyses. Additionally, the expression of both tenogenic and cytokine genes in hTSPCs was influenced by hTGF-β1, indicating that the environment assembled was suitable for studying tendon stem cells differentiation. The study offers insights into the use of 3D cultures of hTSPCs as an in vitro model for investigating their behavior during tenogenic events and opens perspectives for following the potential impact on resident stem cells during regeneration and healing events.

Objective: The purpose of this study was to compare the functional and workers compensation results of displaced intra-articular distal radius fractures treated with three diferent type of treatments.

Material and Methods: A randomized prospective study to evaluate 70 patients with displaced intraarticular distal radius fractures. The mean age were 40 years (range, 22–65 years) and all patients were medium or high level workers (40% dominant-hand). Three randomized groups were treated: 19 patients with close reduction and a cast (group 1); 24 patients with close reduction, percutaneous fixation with Kw and a cast (group 2); and 27 patiens with close reduction and external fixation and Kw (group 3).

Postoperative complications, pain (visual analogue scale), clinical and functional outcome based on DASH score, grip strength, X-ray evaluation, time to return to work and activity level were evaluated and compared at 3, 6 and 12 months of prospective follow-up.

Results: Consolidation was obtained in all cases at 7 weeks. Results at 12 months of follow-up: Pain evaluation score: 2.3 (group 1), 2.9 (group 2) and 1.5 (group 3); mean lost of ROM was 11° (group 1), 11° (group 2) and 23.9° (group 3); mean DASH score was 7 (group 1), 29 (group 2) and 12 (group 3). Average lost of pinch strength was 18.3% (group 1), 23% (group 2) and 35% (group 3). Non-aceptable X-ray parameters: 65% (group 1), 35% (group 2) and 50% (group 3).

Re-operations: 10% (group 1), 7.6% (group 2) and 14.8% (group 3). The average time to return to work (weeks) was 12 (group 1), 14 (group 2) and 19.3 (group 3). All patients return to the work and activity level they had before injury.

Conclusions: Similar results were obtained in the three groups at 12 months but better clinical results for the conservative group (group 1) at 3 and 6 months of FU. The orthopaedic treatment achieved better functional results with lesser lost of ROM, time out of work, more grip strength and better DASH score. Percutaneous fixation achieve better X-ray results at the end of FU with lesser re-operations. The functional and clinical outcomes after one year still are unknown. Hence, more and longer studies are required to confirm these results.

Objective: to study the surgical applicability and clinical results of the Vira^® system in treatment for severe fractures of the calcaneus.

Material and Method: 50 acute intraarticular fractures of the calcaneus treated with the Vira^® system over a two-year period were analyzed in 42 patients. The mean age was 41 years. Eight were bilateral fractures and 47 were closed fractures. According to the Sanders classification, most of the fractures were type IV, followed by type III AB, and the inferior cortical was broken in 42 cases. All the patients were evaluated, in a prospective manner, using the AOFAS scale, plain radiographs and CT scan studies.

Results: the average AOFAS score 12 months after surgery was 76.6 points (SD: 13.9). In 26% cases the results were very good, in 62% good, and in 12% mild and poor. The Böhler angle improvement after surgery was significant (p=0.05) though clinically irrelevant. Subtalar arthrodesis was achieved in all but two cases. The post-surgical complications were: 4 disorders of the plantar support, 4 cases of osteolysis at the tip of the screws, and 3 soft tissue problems.

Conclusions: The Vira system is a validated option for the surgical treatment of severe fractures of the calcaneus, yielding good clinical and radiological results with a surgical procedure that is only minimally aggressive and has a low rate of complications.

Aim: Total Wrist fusion is the main procedure for treatment of postraumatic and degenerative wrist osteoarthritis. During the last decade, midcarpal fusion has become more and more popular as it preserves motion. The purpose of this study was to compare the functional and workers compensation results of both treatments on active workers.

Material and Methods: A prospective study to evaluate 77 patients (76 male and 1 female) who underwent wrist fusion, between 2002 to 2006, with an average of 28 months of follow-up (range, 12–58 months) were made. The mean age were 32 years (range, 25–48 years) and all patients were medium or high level workers with postraumatic and degenerative wrist osteoarthritis. The aethiology in 67% of the patients was SNAC wrist. Right hand was involved in 65% of the patients.

Thirty-eight patients were treated with scaphoid excision and 4-corner fusion using dorsal circular plate. Thirty-nine patients were treated with total wrist fusion using one single, dorsal, precontoured and tapered plate for osteosynthesis and third carpometacarpal joint (CMCJ-3) was included. All patients were immobilised in a cast for 4 weeks after surgery.

Postoperative complications, pain (visual analogue scale), clinical and functional outcome based on Green and O’Brien score, grip strength, X-ray evaluation, time to return to work and activity level were evaluated and compared.

Results: Consolidation was obtained in all cases at 10 weeks. Pain evaluation score was 19,2 (4-corner) and 13,8 (total fusion) on post-op time. The mean modified Mayo wrist score was 70,4 (4-corner) and 69 (total wrist). Average lost of pinch strength was 43% (4corner) and 21% (total fusion). 2 patients with 4-corner fusion required total wrist arthrodesis. Three cases who had a total wrist fusion, required implant removal.

The average time to return to work was 17 weeks (4-corner) and 16,2 weeks (total fusion). All patients return to work. Twelve percent of four-corner fusion and 72% of total wrist fusion return to the same work level with restrictions (until 33% of activity). Twenty-two percent of 4-corner fusion and 28% of total wrist fusion were unable to return to their previous activity level, performing lower intensity work activities. Overall satisfaction was high in both groups with 85% (4corner) and 93% (total fusion).

Conclusion: Both fusion techniques allows an effective stabilization, maintaining the bone stock and eliminate wrist pain with fast return to work.

Total wrist fusion had less surgical failures, better level of satisfaction, lesser lost of force than 4-corner fusion, with less potential for further deterioration with time. However, 4-corner fusion allows return to work with a similar activity level and preserve a functional range of motion in patients with high levels of activity.

Although modern operative intervention for calcaneal fractures has improved the outcome in many patients, there still is no real consensus on treatment, operative technique, or postoperative management. Vira® is a system for reconstruction-arthrodesis of severe calcaneal fractures, consisting in minimally invasive surgery using cannulation technique.

The aim of our study was to elaborate a CPG to assist physiotherapists in decision making and to improve the efficacy and uniformity of care for patients with severe calcaneal fractures.

The CPG was developed according to international methods of guideline development. To identify “best evidence” a structured search was performed. When no evidence was available, consensus between experts (physiotherapist and orthopaedic surgeons) was achieved to develop the guideline. To identify “best clinical experience” and “physiopathology reasoning” focus group of practicing physiotherapists was used. They reviewed the clinical applicability and feasibility of the guideline, and their comments were used to improve it.

CPG include three phases determined from the physiopathology and biomechanical reasoning of surgical system (weeks after the surgery: 2^a–5^a, 5^a–14^a, 14^a–+/−24^a). Unfortunately, evidence related to the treatment of severe calcaneal fracture was sparse and often of poor methodologic quality. The recommendations that were included: early onset (2^a week after the surgery) with early mobility and loading, program of home exercises, manual therapy (articular and miofascial techniques), walking in swimming pool, continuous electromagnetic fields of 99Hz with an intensity of 99 Gaussian during 30 min/day; electrotherapy of the intrinsic muscles of the feet (80Hz; 8:12, 20 mi), a program of active exercises of the feet (dorsiflexion and plantarflexion, not supination and pronation) and resistive exercises of triceps surae muscle (7^a week), criotherapy and anti-inflammatory positions.

Introduction and purpose: The purpose of this study was to assess the functional results of ankle arthrodesis as a treatment of posttraumatic sequelae.

Materials and methods: We carried out a retrospective study from the 1st of January 2000 to the 31st of December of 2005 with a total of 55 patients that underwent posttraumatic ankle arthrodesis, with a mean follow-up of 5 years (range: 1–7 years). The mean age of the patients was 40 years (range: 18–61). There were 52 men and 3 women, the right side (40) predominated over the left (15). Seventy-eight percent of trauma sustained was high-energy with a predominance of fracture of the tibial pilon (type C3 fractures on the AO classification). There were 35% open fractures. The predominant type of surgical technique performed used cannulated screws without any iliac crest grafts. The mean time from surgery to discharge was 9 months (range 4–19).

Results: Repeat arthrodesis was necessary in 16% and an infrapatellar amputation was necessary in 1 case. Only 1 patient returned to their workplace without limitations, the rest were discharged to a medical board for sequelae. The use of crutches/insoles and subjective assessment of pain were related to a lateral tibiotalar angle greater than 90° and varus deformity.

Conclusions: Tibiotalar arthrodesis is a useful rescue technique in patients with severe posttraumatic ankle sequelae. It eliminates pain and increases stability but is the cause of a significant loss of function of the lower limb.

Introduction and purpose: The VIRA system allows reconstruction of the fractured bone and its arthrodesis with the talar bone eliminating all movement of the subtalar joint by means of a minimally invasive approach. The aim is to restore the shape of the foot and prevent joint degenerative changes in the long term without the complications and sequelae of open surgery.

Material and methods: Between November 2004 and June 2006, 30 intraarticular calcaneal fractures in 25 patients were treated surgically by means of the Vira System. Using Sanders’ classification 6 cases were classified as type II, 13 as type III and 11 as type IV. Five patients had open fractures. There was a mean period of 8.7 days between the accident and surgery. Seven cases had associated lesions and fractures in other locations. Only 2 cases required iliac crest grafts in the operated area.

Results: All patients except 10 have returned to their usual occupations. Eight cases evolved favorably but have had a short follow-up. Two cases experienced a delayed healing of the subtalar arthrodesis and had to be reoperated; they required an autologous graft. The mean period of temporary disability in patients discharged from hospital was 163.7 days. Clinical assessment using the AOFAS scale reached a mean value of 76.6 points. No surgical or post-surgical complications were seen in the group studied.

Conclusions: The VIRA System seems capable of achieving its purpose in the first series of patients operated. It allows a quick recovery both postoperatively and in the subsequent evolution without any associated complications. It accelerates the patients’ return to work by decreasing convalescence and minimizing sequelae.

Introduction and purpose: The non-reestablishment of normal scaphoid anatomy is related to instability, loss of function and secondary arthritis. The most appropriate treatment for unstable non-unions with scaphoid deformity is the use of a trapezium-shaped bone graft and internal fixation. We present our experience and the results obtained by treatment of this condition.

Materials and methods: We retrospectively studied 35 patients (34 males and 1 female) with unstable scaphoid non-union treated by curettage, trapezium-shaped iliac crest bone graft and internal fixation, with a mean follow-up of 24 months (range: 12–52 months). Mean age of the patients was 29.8 years (range: 18–52) and all had medium to high functionally demanding jobs. In 48% of cases it was the dominant wrist that underwent surgery. In 3 cases there was a previous history of union of scaphoid fracture. To fixate the graft different devices were used: metal cannulated screws (54%), Kirschner wires (22%) and biodegradable screws (22%). Growth factors were used in 5 cases (PRP) and the wrist was immobilized for 8 weeks. We assessed complications, range of movement, union on x-ray, Green and O’Brien’s scale and return to work at the end of follow-up.

Results: Complete union was obtained in 68.6%, partial in 14.3% and incomplete in 17.1%. Four patients (13.8%) required repeat surgery: 2 four-corner arthrodesis, 1 a conventional graft and 1 a vascularized graft. The mean flexion-extension arc was 99.6°; radio-ulnar deviation was 48.6°. According to the modified Green-O’Brien scale, 93% of the patients had excellent to good results. Time off work after surgery was 21 weeks (9-50 weeks). Ninety-five percent of patients returned to their former job. There were no statistically significant differences with reference to the location of the non-union of the fixation used, although cannulated tapered screws had a lower union rate and a greater number of complications. The worst results were associated with previous surgery and signs of radiocarpal degeneration.

Conclusions: Restoration of normal scaphoid morphology by means of a trapezium-shaped graft results in good outcomes and allows an optimum return to work in manual workers. Previous surgery, signs of radiocarpal degeneration and voluminous implants cause the worst functional results. Even if complete union is not seen on x-rays, the functional result may be optimum.

The hallux rigidus, first described by Nicoladoni in 1881 (1), is the painful and decreased motion, especially dorsiflexion at the metatarsophalangeal joint, of the great toe. The purposes of this report were to evaluate the mid-term results of the Sliding osteotomy technique(2,3,4).

Thirty nine (46 feet) consecutive patients (mean age 38 years) with hallux rigidus of I and II grade were followed over a five-year period.

All patients were evaluated clinically and radiographically preoperatively and post operatively.

At the time of final follow-up, the mean AOFAS score was significantly improved: excellent 26 (56,5%); good 12 (26%); fair 6 (13%); poor 2 (4,5%).

This clinical review suggest that this procedure as a safe, effective measure to treat in patients with hallux rigidus of I and II grade.

Introduction: Intraarticular fractures of distal radius are associated with high energy mechanisms, are severe and difficult to obtain a surgical reduction. The aim of our paper is to compare the clinical, radiographically and activity results in workers treated with surgical and conservative procedures.

MATERIAL AND METHODS: A prospective study was organized in 43 heavy-labour workers (34 male and 9 female) with unstable fractures of distal radius, treated between 2003 and 2005. The minimal follow-up was of one year. The mean age were 40 years (22–65 years) and dominant limb was involved in 40% of the cases. To treatment groups were established. Group 1, conservative treated with indirect reduction and cast immobilization (n=20) and Group 2 surgically treated with indirect reduction and percutaneous fixation with K-wires and cast immobilization (n=24).

Pain, mobility and radiograhs were evaluated and also strength (isokinetics), functionality (DASH score) and, finally, the return to work at 3, 6 and 12 months.

RESULTS: Fracture healing was obtained at 7 weeks but the time to return to work were 14 weeks after surgery. Pain score, at 12 months, were 2,3 points for conservative treatment and 2,9 points for the surgically group. The flexion – extension mobility loss, in relation to the contralateral wrist, was lesser in the conservative group at 3 and 6 month but similar (11°) at 12 months in both groups. Radiographs corrections were anatomically in 38% of the cases of group 1 and in 80% of group 2. Functional and strength results were similar in both groups. All the patients return to the same work activity.

CONCLUSIONS: Percutaneous fixation of unstable intraarticular distal radius fracture is comparable to the conservative treatment but the percentage of anatomical reductions was higher. It would be of importance in the evolution of the patients.

AIM: The scaphoid resection with four-corner fusion is an effective procedure for treatment of postraumatic and degenerative wrist osteoarthritis. Few studies that evaluated the functional and workers compensation results are available in the literature. We presented the results of 4-corner fusion on active heavy labour workers.

MATERIAL AND METHODS: A prospective study to evaluate 38 patients (37 male and 1 female) who underwent 4-corner fusion, between 2002 to 2005, with an average of 24 months of follow-up (range, 12–48 months) were made. The mean age of the patients were 32 years (range, 25–48 years). All patients were heavy-labour workers. The aethiology in 77% of the patients was SNAC wrist. Dominant limb was involved in 65% of the patients. All patients were immobilised in a cast for 4 weeks after surgery. Patients were assessed clinically and radiographically. Functional analysis of grip and pinch strenght were performed.

RESULTS: Consolidation was obtained in all cases at 10 weeks. Pain evaluation score was 77 at pre-op and 19 on post-op time. The range of flexion – extension movement postoperative was 57°. Average lost of pinch strength was 25%. All patients return to work, 80% to the same activity level and 20% to a different work performing less strenuous activities. After surgery, the range time to return to work was 160 days.

CONCLUSION: The four-corner fusion allows an effective stabilization, maintaining the bone stock and eliminate wrist pain. This technique permit a fast return to work with a great level of satisfaction and preserve a functional range of motion with a minimum lost of force in heavy labour workers.

AIM: We present the results of scaphoid non-unions treated with open reduction, bone grafting and internal fixation with biodegradable implants on active heavy labour workers.

MATERIAL AND METHODS: Between 2002 to 2004, 20 patients with scaphoid non-unions were treated by open reduction, bone grafting and internal fixation using self-reinforced poly-L-lactic acid screws. The mean prospective follow-up was 24 months (range, 12–38 months). The mean age was 28 years (range, 18–42 years). All patients were male and heavy-labour workers.

The patients were assessed clinically (modified Mayo wrist score) and radiograhically. The grip and pinch strength were also studied.

RESULTS: We find 13 excellent results, good in 5 cases and poor in 2 cases. A Matti-Russe group patient was revised 6 months after the first intervention. Any fragment displacement, implant loosening or adverse reaction was found. Most of the patients (90%) return to the same work and the same level without complications.

CONCLUSION: Both groups are a good alternative for the scaphoid non-unions treatment. However, biodegradable implants disappears in the time, the removal are no necessary, facilitate the revision surgery, if necessary, and permitted MRI studies to evaluated the graft viability.

Background: Recurrent peroneal tendon subluxation is uncommon. We report the results of a delayed anatomic repair using suture anchors. Using a case series we tested the null hypothesis that there are no differences between pre- and post-operative status following anatomical repair of the superior peroneal retinaculum.

Methods: In the period 1996 to 2001, we operated on 14 patients (all males; average age 25.3 ± 6.3 years, range 18–37) with traumatic recurrent unilateral peroneal tendon subluxation, with a followed up of 38 ± 3 (range 22 to 47) months.

Results: No patient experienced a further episode of peroneal tendon subluxation, and all had returned to their normal activities. Maximum calf circumference, functional ability, peak torque, total work and average power of plantar flexion were always lower in the operated leg, but the differences did not reach statistical significance. The AOFAS Ankle-Hindfoot Scale increased significantly from 54.3 ± 11.4 to 94.5 ± 6.4 (p = 0.03), with five patients reporting a fully normal ankle.

Conclusion: If an anatomic approach to treating the pathology is utilised, reattachment of the superior retinaculum is a most appropriate technique. It returns patients to a high level of physical activity, and gives high rate of satisfactory results both objectively and subjectively. Randomised control trials may be the way forward in determining the best surgical management method. However, the relative rarity of the condition and the large number of techniques make such a study difficult.

Objective: To report the outcome of surgery for chronic recalcitrant Achilles tendinopathy in sedentary and athletic subjects.

Design: Case control study

Participants: We matched each of the 61 non-athletic patients with a diagnosis of tendinopathy of the Achilles tendon with an athletic patient with tendinopathy of the main body of the Achilles tendon of the same sex who was within two years of age at the time of operation. A match according was possible for 56 patients (23 males and 33 females). 48 sedentary subjects and 45 athletic subjects agreed to participate.

Main Outcome Measure: Outcome of surgery, return to sport, complication rate.

Results: Non-athletic patients were shorter and heavier than athletic patients. They had greater BMI, calf circumference, side-to-side calf circumference differences, and subcutaneous body fat than athletic patients. Of the 48 sedentary patients, only 25 reported an excellent or good result. Of these, three had undergone a further exploration of the Achilles tendon. The remaining patients could not return to their normal levels of activity. In all of them, pain significantly interfered with daily activities.

Conclusions: Non-athletic subjects experience more prolonged recovery, more complications, and a greater risk of further surgery than athletic subjects with recalcitrant Achilles tendinopathy. Key words: Achilles tendinopathy, surgery.

Introduction and purpose: When treating for loss of mobility in the elbow, after at least six months of conservative treatment following an injury we should consider surgery. The purpose of this study is to evaluate the improvement in range of motion after surgery.

Materials and methods: We reviewed the cases of elbow arthrolysis carried out in our hospital from 1999 to 2004, analysing the following variables: personal data (sex, age, type of work) and information on the injury (type, location, associated lesions and degree of stiffness). The main variables were degree of mobility in flexion/extension (F-E) and pronation/supination (P-S) before and after surgery, in addition to functional and occupational results.

Statistical analysis: Percentage estimate and by 95% confidence intervals, and analysis of increased mobility after surgery in F-E and P-S using Student’s t-test of repeated measures.

Results: 52 patients who underwent arthrolysis (86% males, mean age 37.2 years) whose jobs required average exercise and with grade II stiffness in 46.2% of the cases. The average preoperative ranges of motion for F-E and P-S were 74.52 (SD 32.3) and 120.10 (SD 66.6) degrees, respectively. The postoperative estimates for both parameters were 96.5 (SD 29.5) for F-E and 158.9 (SD 39.8) for P-S. The increases were statistically significant (p< 0.00001). The increased motion in P-S was slightly better than for F-E (p=0.054).

Conclusions: After our study, we can confirm that arthrolysis is an effective surgical procedure to improve mobility in stiff elbows. It is indicated when the joint interline is preserved. The lateral approach is the most common because it enables access to the anterior and posterior aspects of the capsule. Good functional and occupational outcome in a high percentage of cases.

Purpose: To assess the mid-term result of untreated stable longitudinal tears diagnosed during ACL reconstruction surgery.

Materials and methods: The study comprised 19 patients who had peripheral longitudinal tears of the internal meniscus and 6 with longitudinal tears in the external meniscus, diagnosed during ACL reconstruction surgery. No surgical procedure was applied to these tears. In 14 of the 25 patients, the ACL was reconstructed using RIGIDFIX (Mitek) and in 11 the SWIN-BRIDGE (Citieffe) was used. The mean follow-up was 20.5 months. The clinical evaluation was made using the IKDC score.

Results: None of the 25 patients had to be revised for meniscal problems in the two years after surgery. Three of them were subjected to minor revisions (mobilizations under anesthesia). All patients went back to work after an average of 3.2 months after surgery. 4 patients were classified as belonging to class A in the IKDC score, 16 fell into class B and 5 into class C. None of the patients was rated as belonging to class D.

Conclusions: Conservative treatment of stable longitudinal meniscal tears diagnosed during ACL surgery yields good mid-term results. The number of revisions due to meniscal problems is very small.