Tibial and femoral bone tunnel widening (TW) has been observed following anterior cruciate ligament (ACL) reconstruction. We developed a χ12 mm cannulated cancellous screw (Intercondylar Ligament Screw, ICLS) for femoral fixation to reduce TW.

The purpose of this study is to introduce our surgical method and its results. We employed an original ICLS system developed to reduce the needed distance between the tibial and femoral-fixation points (distance between fixation points, DbF) in ACL reconstruction. Five-strand (sometimes four or six-strand) hamstring grafts are connected to the ICLS. Tibial fixation is achieved with a Ligament Tension Screw, which had been developed by Murase et al. rom 2001 to 2008, 169 knees underwent ACL reconstruction at our hospitals using our ICLS system. TW was evaluated by radiographs at least three months postoperatively. An enlargement of more than 2 mm was considered TW. The following was also evaluated: range of motion, the limb symmetry index (LSI, injured leg divided by uninjured and multiplied by 100), value of knee extension power in OKC, anterior knee laxity, Lysholm score, and DbF. The average length of DbF was 38.1 mm (n=132). Only 6.7% (n=104) of cases showed more than 2 mm of TW. Mean LSI was 83.3%(n=77) four months postoperatively. The mean Lysholm score was 96.2(n=68) at three months after ACL reconstruction. The mean side-to-side difference in anterior tibial translation, measured with use of a KT-2000 or Knee Lax, was 1.60 mmï1/4N=57ï1/4‰.

We were able to reduce TW after ACL reconstruction using our ICLS system with good results.

Graft fixation in anterior cruciate ligament reconstruction is a basic criterion for the outcome of the surgical procedure. Several solutions have been proposed; each of them had advantages and disadvantages, and the choice of a surgical technique often represents the surgeon’s opinion.

The goal of the fixation is stability and incorporation of the graft in the bone tunnels. Bone-patellar tendon-bone graft has the advantage of bone to bone fixation, which is impossible using tendon grafts. Femoral fixation techniques for hamstrings can be classified as follows: compression techniques, expansion techniques and suspension techniques (cortical or cancellous).

Top Traction System (TTS) is a new technique for femoral fixation using hamstrings. Here, a retrograde screw is fixed to the anterolateral cortex of the femur. A ring is fixed to the screw to pass the tendons.The screw is self-threading, 28 mm long and 6.5 mm in diagmeter; its pull-out strength is 1350 N. The screw is made from a titanium alloy and only available in one size.

Surgical technique is simple and reproducible and the instruments are easy to use. The positioning of the screw is fully guided by instruments that minimise errors.

With this fixation device further graft traction is possible after tibial fixation without twisting the hamstrings. Results are good at the short-term evalutation but a long-term follow-up is required before a final recommendation can be made.

Aim: The aim of our study was to evaluate the results of the Soffix Mark I and Mark II hamstring fixation devices, placed transtibially with an “over the top” femoral route when applied to revision anterior cruciate ligament (ACL) surgery secondary to synthetic ligament failure.

Patients and Methods: 29 ACL revisions performed between 1992 and 2000 were evaluated. Twenty six failed prosthetic ligaments, 2 failed semitendinosus/gracilis (STG) and one BTB autografts were revised using hamstring grafts in 26, quadriceps in 2, and patella bone tendon bone (BTB) in 1 patient. Mark I and II fixation devices were employed. Follow up included clinical examination, KT 2000 arthrometric assessment, Lysholm, Tegner and IKDC scoring. The average follow up time was 50 months ±22.4.

Results: Arthrometric examination showed a mean side to side difference (SSD) of 2.98 mm ±1.5. The mean Lysholm score was 86.5 ±10.5 and 21 patients had a B rating (nearly normal) on IKDC scoring, while 3 score A (normal). The mean Lysholm score was 86.5 ±10.5. The lowest clinical scores were noted in 4 multiply operated knees.

Conclusions: We concluded that a revision technique using the STG Soffix fixation device can restore stability with good functional outcomes following failed primary ACL reconstruction. Multiply re-operated knees had the worst functional results despite restoration of stability.

To investigate whether the harvesting of Hamstring graft in ACL reconstruction results in compromised knee flexion strength and proprioception, and hence knee function?, a prospective study, approved by the local Ethics Committee, to assess the function and strength of the knee joint in patients who had ACL reconstruction done using a four-strand Hamstring graft. The control group was the contra lateral knees. 28 knee joints were studied with mean follow-up of 70.1 weeks (52–156). All operated knees received an extensive set regime of pre-and post-operative physiotherapy. Assessment tools were clinical examination, Laxometer arthrometry for measured anterior draw, Biodex dynamometry and Stabilometry for Hamstring and quadriceps strength and proprioception. The knee function was assessed using a questionnaire incorporating IKDC (International knee documentation committee) performa, Lysholm 2 score, Tegner’s activity scale and Oxford knee score.

Following reconstruction (mean 70.1 weeks postop), objective assessment using Biodex dynamometer showed that mean peak flexion torque around the knee joint was 69.8 N-m and 76.2 N-m in the operated and non-operated knee respectively. There was no difference in flexion torque in both groups. Mean Flexion: Extension ratio around the knee joint was 53.9% in the operated and 53.2% in non-operated sides. Mean stability index, measured using open eye stabilometry, was 3.5 (SD 2.4) in the operated side and 3.1 (SD1.8) in the non-operated side, with no significant difference demonstrable (p< 0.05).

The mean age of patients was 28.3 years (18–44). Mean IKDC score following reconstruction was 74.8 (49–100), SD18.5. Mean Tegner’s activity scale improved from 2.5(3–7) pre-operative to post-operative 5.4(3–7), p< 0.01. Mean Lysholm 2 score improved from 53.4(41–76) pre-operatively to 85(64–100) post-operatively, p< 0.01. Subjective function of the knee on a scale of 0–10 improved from pre-operative 3.1 to post-operative 7.7 (p< 0.01). Arthrometry at 25-degree flexion and 130 N force using Laxometer showed mean anterior laxity 5.3mm on the operated side and 3.1 on the healthy side (side to side difference 2.2mm).

Conclusion: The function of the knee improved significantly following ACL reconstruction both objectively and subjectively. The harvesting of Hamstring as a graft neither compromises the flexion torque nor the proprioception around the knee joint.

ACL (anterior cruciate ligament) partial tears include various types of lesions, and an high rate of these lesions evolve into complete tears. Most of the techniques described in literature for the surgical treatment of chronic partial ACL tears, don't spare the intact portion of the ligament. Aim of this study was to perform a prospective analysis of the results obtained by augmentation surgery using gracilis and semitendinosus tendons to treat partial sub-acute lesions of the ACL. This technique requires an “over the top” femoral passage, which enables salvage and strengthening of the intact bundle of ACL. The study included 97 patients treated consecutively at our Institute from 1993 to 2004 with a mean injury-surgery interval of 23 weeks (12–39). Patients were followed up by clinical and instrumental assessment criteria at 3 months, 1 year and 5 years after surgery. Clinical assessment was performed with the IKDC form. Subjective and functional parameters were assessed by the Tegner activity scale. Instrumental evaluation was done using the KT-2000 instrument: the 30 pound passive test and the manual maximum displacement test were performed. We obtained good to excellent results in 95.9% of cases. We didn't observed recurrences in ligamentous laxity. We believe that the described technique has the advantage of being little invasive, compatible with the ACL anatomy, and enables very rapid functional recovery and return to sport.

Introduction: Mechanical factors are thought to be one of the main reasons in determining tunnel enlargement after ACL reconstruction with hamstrings. The purpose of this prospective study was to evaluate how the different techniques may affect the bone tunnel enlargement.

Material and Method: Forty-five consecutive patients undergoing ACL reconstruction with the use of autologous doubled semitendinosus and gracilis tendons entered this study. They were randomly assigned to enter group A (In-Out technique, with cortical fixation and Interference screw) and group B (Out-In technique, metal cortical fixation on the femour and tibia). At a mean follow-up of 10 months, all the patients underwent clinical evaluation and a CT scan exam to evaluate the post-operative diameters of both femoral and tibial tunnels.

Results: The mean femoral tunnel diameter increased significantly from 9.05±0.3 mm (post op) to 10.01±2.3 mm (follow-up) in group A and from 9.04±0.8 mm to 9.3±1,12 mm in group B. The mean tibial tunnel diameter increased significantly from 9.03±0.04 mm to 10,68±2.5 mm in group A and from 9.04±0.03 mm to 10.±0,78 mm in group B. The mean increase in both femoral and tunnel diameters observed in group A was significantly higher than that observed in group B (p< 0.05). Stability evaluated with kt 1000 don’t significantly differ in the two groups

Conclusion: The results of this study suggest that different angular orientation techniques and different hardware devices may affect tunnel enlargement after hamstrings reconstruction. The reason can be reached from the different stiffness of the devices and their backlashes on the tunnels walls.

The chioce of the graft and its fixation in LCA reconstruction is basic for the outcome of the surgical procedure. Several solutions have been proposed; each of them had advantages and disavantages. The choice of the graft and the surgical technique is often due to surgeon’s opinion.

The goal of the fixation is stability which allowes incorporation of the graft in the bone tunnels. Bone-patellar tendon-bone graf has the advantage of bone to bone fixation which is impossible using tendon grafts. Femoral fixation techniques for hamstrings can be classified in: compression tecniques, expansion tecniques and suspension tecniques (cortical or canellous).

TTS (Top Traction System) is a new tecnique for femoral fixation using hamstrings. The fixation is achieved by a retrograde screw that allowes fixation to the antero-lateral cortex of the femur. A ring is fixed to the screw to allow tendons passing. The screw is sefl-threading, its lenght is 28 millimeters and its diameter is 6,5 millimeter; its pull-out strenght is 1350 N. The screw is built in titanium alloy and only one size.

Surgical technique is simple an reproducible and the instruments are easy to use. The positioning of the screw is fully guided by instruments that minimize errors.

This fixation device allowes further graft traction after tibial fixation without twisting hamstrings. Results after 5 years are good but we need long term follow-up for final opinion.

Background Female patients undergoing arthroscopic anterior cruciate ligament reconstruction with hamstring tendon graft developed increased post-operative laxity compared to male and female patients who had been reconstructed using patellar tendon graft.

Hypothesis Supplementary tibial fixation in female patients will reduce laxity.

Study Design Prospective, randomized, double-blinded clinical trial.

Methods Fifty-six female patients divided into two groups (standard tibial fixation versus supplementary staple fixation) were followed for 2 years.

Results After 2 years the mean side-to-side difference utilizing KT-1000 arthrometer manual maximum measurements was 1.8 mm (standard group) and 1.1 mm (staple group) (p=0.05). A Grade 0 Lachman test was present in 63% of the standard group and 86% of the staple group (p=0.04). Kneeling pain was experienced by 7% of the standard group and 29% of the staple group (p=0.05).

Conclusions Supplementary tibial fixation in female patients undergoing ACL reconstruction with hamstring tendon graft and interference screw fixation with a single screw size significantly improves laxity measurements and clinical stability assessment 2 years postoperatively. However, this is at the cost of increased kneeling pain.

Purpose: To investigate sciatic nerve conduction during hamstring lengthening.

Conclusion: Sciatic nerve traction is caused during hamstring lengthening.

Summary of method, results, and discussion: Ten children with spastic cerebral palsy underwent distal hamstring lengthening, average popliteal angel before surgery was 80 degrees.

Methods: The tendon of the semitendinosus was elongated by sliding lengthening. The gracilis tendon was cut and the tendons of the biceps and semimembranosus were elongated by dividing the aponeurosis. Thereafter to elongate the hamstring the hip and knee were flexed to 90 degrees and the knee slowly extended with continuous evoked EMG monitoring. Bipolar nerve stimulation placed near the sciatic nerve consisted of the delivering of rectangular impulses of amplitude 0.8-1.2 ma for 100 US duration. The EMG recordings were performed from the tibialis anterior muscle.

Results: In all patients motor potential amplitude gradually decreased during extension of knee (hamstring lengthening). The average decrease of the amplitude at popliteal angle of 60 degrees was 37 percent (16-75) and at 30 degrees 83 percent (36-98). The elongation was stopped at 30° of popliteal angle. On extending the hip and knee motor potential amplitude returned to normal. Discussion: Elongation of hamstring muscle is associated with traction on the f sciatic nerve as appears by decrease in sciatic nerve motor potential amplitude. To avoid nerve injury no excessive hamstring lengthening should be done and no nerve traction should be allowed at postoperative immobilization.

Aims: The purpose of the paper is to compare results of reconstruction of ACL with quadrupled ST and GR tendons via arthrotomy in the study group and arthroscopy in the control group.

Methods: Treatment results of the consecutive cohort of 26 patients in the study group treated by arthrotomy were compared to the results of the consecutive cohort of 22 patients in the control group treated by arthroscopy operated between September 2002 and December 2003 by the same operating team. The patient’s age varied from 17 to 46 years (mean 24,5y) in the study group and 18 to 48 years (mean 23,7y) in the control group, with minimum follow-ups longer than 30 months. For the evaluation clinical examination, Lysholm& Gillquist and Marshall scales and KT-1000 assessment were used. For the proximal fixation endo-button plate and for the distal screw-post fixation were applied. There were no differences in graft harvesting, graft preparation and rehabilitation protocol in both groups.

Results: There were 3 traumatic graft failures in the study group. Mean gained Lysholm& Gillquist score in the study group was 31,42(+−4,68SD) and mean gained Marshall score was 12,18(+−2,27SD). 57.69% of the patients in the study group returned to the sport and work without any limitation on the preoperative level, additional 30.76% has only minor limitation in sport or work. There was 1 case of superficial infection and 1 deep infection in the study group. Both infections resolved without sequels. MM lesions in 10 cases (38,46%) and no LM lesions were found intraoperatively.

There were 2 traumatic and 1 unclear graft failures in the control group. Mean gained Lysholm& Gillquist score was 34,35(+−8,86SD) and mean gained Marshall score was 12,33(+−2,88SD). 45,46% of the patients in the control group returned to the unlimited sport and work on the preoperative level, additional 36,37% has minor limitation in sport or work and 1 patient despite stable knee was unable to return to the work. There was 1 case of deep vein thrombosis of the operated leg and 1 neuroma in the arthroscopy portal area in the control group. MM lesions in 12 cases (54,5%) and LM lesions in 5 cases (22,7%) were found intraoperatively.

The average KT-1000 side-to-side difference in the study group was 2,88 mm and in the control group 2,73 mm.

Conclusions: Mid-time follow-up reveals good to very good clinical outcome in both groups with no statistically significant differences. It looks interesting why the better average return to sport ratio was gained in the study group. 2 cases of infection discourage this approach except necessity additional cartilage repair. Differences in meniscal injuries rate might also advocate for arthroscopy as more capable in diagnose and treatment.

Anterior cruciate ligament injury is the most common and economically costly sport injuries, frequently requiring expensive surgery and rehabilitation. Post-operative knee septic arthritis represents a serious complication with an incidence rate between 0.14% and 1.7%. A common practice to avoid septic arthritis is the “vancomycin wrap”, consisting in the soaking of the graft for 10–15 minutes within a sterile gauze swab previously saturated with 5 mg/mL vancomycin. Even though several studies have been conducted to investigate vancomycin toxicity on different musculoskeletal tissues or cells, little is known about the effect of such antimicrobial on tendon-derived cells.

The aim of this study was to determine the in vitro toxicity of different concentrations of vancomycin at different time points on human primary tenocytes (hTCs).

hTCs were isolated from hamstring grafts of patients undergoing anterior cruciate ligament reconstruction. After expansion, cells were treated with different concentrations of vancomycin (2.5, 5, 10, 25, 50 and 100 mg/mL) for 10, 15, 30 and 60 minutes. In vitro toxicity was evaluated measuring: metabolic activity through the reduction of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT Assay); cytotoxicity (Live/Dead assay); and cell apoptosis (Annexin V apoptosis kit).

The metabolic activity of hTCs was affected by vancomycin treatment starting from 10 mg/mL at all time points (p < 0.05) and dropped down at 100 mg/mL at all time points (0.05 < p < 0.001). Cells viability resulted to be unaffected only by 2.5 mg/mL vancomycin at all time points. Vancomycin resulted to be cytotoxic starting from 10 mg/mL after 15 minutes of treatment and at all higher concentrations under study at all time points. Cells died when treated with vancomycin concentrations higher than 5 mg/mL but not through apoptosis, as confirmed by negative staining for Annexin V.

In our experimental conditions, vancomycin resulted to be toxic on hTCs at concentrations higher than 5 mg/mL. The use of this antibiotic on tendons to prevent infections could be useful and safe for resident cells if used at a concentration of 2.5 mg/mL up to 1 hour of treatment.