Aim: This study investigated the difference in proximal tibial cortical strain distribution using a fixed or mobile bearing design for TKA. Methods: Eight fresh frozen human cadaver tibias were used. The strain magnitude and distribution on the anterior cortex of the proximal tibia during axial and rotational loading of the knee were measured with a quantitative full-field strain measurement technique (Electronic Speckle Pattern Interferometry). First, strain distributions of the intact knee were acquired. Subsequently, strain distributions after implementation of conventional and mobile bearing PCL retaining total knee implants (Scorpio®) were measured. Results: Under each loading condition, the minimum principal strain was greater in magnitude as compared to the maximum principal strain. Under 1′500 N axial loading, the resulting minimum principal strain magnitude and orientation was nearly identical between the mobile bearing configuration (500 ± 287 με), and the fixed bearing configuration (500 ± 286 μ ε). In response to 10° internal rotation, this strain increased to 782 ± 371 μ ε and 1000 ± 389 μ ε for the mobile and fixed tibial component, respectively. In response to 10° external rotation, minimal principal strain decreased to 421 ± 233 μ ε for the mobile bearing, but increased to 632 ± 293 μ ε for the fixed bearing. These differences between mobile and fixed bearing scenarios were statistically highly significant. Conclusion: For this in-vitro study under exact controlled loading conditions the mobile bearing design induced less strain in the proximal tibia as the fixed bearing tibial component. The difference in strain levels may be of importance to understand bone remodeling and osseointegration

INTRODUCTION:. The purpose of this study was to determine if a short femoral stem (Lima Corporate, Udine, Italy) would result in a strain distribution which mimicked the intact bone better than a traditional length stem, thereby eliminating the potential for stress-shielding. METHODS:. A 2 mm thick moldable plastic (PL-1, Vishay Micromeasurements, Raleigh, NC) was contoured to six fourth-generation composite femoral bones (Pacific Research Laboratories, Vashon, WA). The intact femurs were then loaded (82 kg) in a rig which simulated mid-stance single limb support phase of gait (Figure 1). During testing, the femurs were viewed and video recorded through a model 031 reflection polariscope. Observing the photoelastic coating through the polariscope, a series of fringes could be seen, which represented the difference in principal strain along the femur. The fringes were quantified using Fringe Order, N, as per the manufacturers technical notes. In order to analyze the strain distribution, the femur was separated into 6 zones, 3 lateral and 3 medial, and the maximum fringe order determined. Upon completion of testing of the intact femur, the short length femoral stem was inserted and tested, and finally the traditional length femoral stem was inserted and tested. Anterior and lateral radiographs were obtained of the femur with each femoral stem in order to confirm proper alignment. RESULTS:. Fringes formed in a similar pattern for all femurs, intact and with stems. The fringes first occurred medially and laterally in a proximal-distal direction and radiated outward, decreasing in fringe order, toward the neutral axis of bending (anterior and posterior). The magnitude of the fringe order, N, remained the same or increased in the proximal to distal direction. This became more prominent, particularly on the lateral side, with the traditional length femoral stem, when a distal migration of the fringes was seen compared to the intact femur (Figures 2 and 3). Medially, with the traditional length femoral stem, the fringes remained but were of a lower magnitude than the intact femur. The femoral strain pattern, resulting from implantation of the short length femoral stem, was found to closely match the intact femur. X-rays confirmed proper alignment of all implants. CONCLUSIONS:. The distal migration of strain seen with the traditional length femoral stem was indicative of potential stress shielding. As an alternative, this study suggests that the short length femoral stem most closely replicates the strain distribution of the intact femur and may limit this type of failure

This study investigated the difference in proximal tibial cortical strain distribution using a fixed or mobile bearing design for TKA. Eight fresh frozen human cadaver tibias were used. The strain magnitude and distribution on the anterior cortex of the proximal tibia during axial and rotational loading of the knee were measured with a quantitative full-field strain measurement technique (Electronic Speckle Pattern Interferometry). First, strain distributions of the intact knee were acquired. Subsequently, strain distributions after implantation of conventional and mobile bearing PCL retaining total knee implants (Scorpio®) were measured. Under each loading condition, the minimum principal strain was greater in magnitude as compared to the maximum principal strain. Under 1,500 N axial loading, the resulting minimum principal strain magnitude and orientation was nearly identical between the mobile bearing configuration(500 ± 287m;e;), and the fixed bearing configuration (500 ± 286m;e;). In response to 10° internal rotation, this strain increased to 782 ± 371m;e; and 1000± 389m;e; for the mobile and fixed tibial component, respectively. In 10° external rotation, minimal principal strain decreased to 421 ± 233m;e; for the mobile bearing, but increased to 632 ± 293m;e; for the fixed bearing. These differences between mobile and fixed bearing scenarios were highly statistically significant. For this in-vitro study under exact controlled loading conditions the mobile bearing design induced less strain in the proximal tibia than the fixed bearing tibial component. The difference in strain levels may be of importance for bone remodeling and osseointegration

Total shoulder arthroplasty is a well-tested procedure that offers pain relief and restores the joint function. However, failure rate is still high, and glenoid loosening is pointed as the main reason in orthopedic registers. In order to understand the principles of failure, the principal strain distributions after implantation with Comprehensive® Total Shoulder System of Biomet® were experimental and numerically studied to predict bone behavior. Fourth generation composite left humerus and scapula from Sawbones® were used. These were implanted with Comprehensive® Total Shoulder System (Biomet®) with a modular Hybrid® glenoid base and Regenerex® glenoid and placed in situ by an experienced surgeon. The structures were placed in order to simulate 90º abduction, including principal muscular actions. Muscle forces used were as follows: Deltoideus 300N, Infraspinatus 120N, Supraspinatus 90N, Subscapularis 225N. All bone structures were modeled considering cortical and the trabecular bone of the scapula. The components of prosthesis were placed in the same positions than those in the in vitro models. Geometries were meshed with tetrahedral linear elements, with material properties as follows: Elastic modulus of cortical bone equal to 16 GPa, elastic modulus of trabecular bone equal to 0.155 GPa, polyethylene equal to 1GPa and titanium equal to 110 GPa. The assumed Poisson's ratio was 0.3 in all except for polyethylene where we assumed a value of 0.4. The prosthesis was considered as glued to the adjacent bone. The finite element model was composed of 336 024 elements. At the glenoid cavity, the major influence of the strain distributions was observed at the posterior-superior region, in both cortical and trabecular bone structures. The system presents critical region around holes of fixation in glenoid component. At the trabecular bone, the maximum principal strains at the posterior-superior region ranged from 2250 µε to 3000 µε. While at the cortical bone, the maximum principal strains were 300 µε to 400 µε. The results observed evidence some critical regions of concern and the effect of implant in the bone strains mainly at the posterior-superior region of the glenoid cavity is pronounced. This indicates that this region is more affected by the implant if bone remodeling is a concern and it is due to the strain-shielding effect, which has been connected with loosening of the glenoid component

Summary Statement. An MRI-derived subject-specific finite element model of a knee joint was loaded with subject-specific kinetic data to investigate stress and strain distribution in knee cartilage during the stance phase of gait in-vivo. Introduction. Finite element analysis (FEA) has been widely used to predict the local stress and strain distribution at the tibiofemoral joint to study the effects of ligament injury, meniscus injury and cartilage defects on soft tissue loading under different loading conditions. Previous studies have focused on static FEA of the tibiofemoral joint, with few attempts to conduct subject-specific FEA on the knee during physical activity. In one FEA study utilising subject-specific loading during gait, the knee was simplified by using linear springs to represent ligaments. To address the gap that no studies have performed subject-specific FEA at the tibiofemoral joint with detailed structures, the present study aims to develop a highly detailed subject-specific FE model of knee joint to precisely simulate the stress distribution at knee cartilage during the stance phase of the gait cycle. Method. A detailed three-dimensional model of a healthy human knee was developed from MRI images of a living subject, including the main anatomical structures (bones, all principal ligaments, menisci and articular cartilages). The femur, tibia and fibula were considered as rigid bodies, while the menisci and articular cartilage were modelled as linearly elastic, isotropic and homogeneous while the ligaments were considered to be hyperelastic. Loading and boundary condition assignment was based on the kinematic and kinetic data recorded during gait analysis. Ten time intervals during the stance phase of gait were separately simulated to quantify the time–dependent stress distribution throughout the cycle from heel-strike to toe-off. Loading condition of the tibiofemoral joint varys during the gait cycle since the joint angle changes from extension to flextion, therefore different joint angles at relative time interval were determined to accurately simulate the varing loading condition. Results. The compressive stress and tensile strain distributions in the femoral cartilage, tibia cartilage and menisci of each selected time interval during the stance phase of gait cycle were quantified and corresponded to specific amount of varus/valgus knee moment obtained by inverse dynamics analysis of the kinematic and kinetic data from gait analysis. Therefore a correlation between stress/strain and the frontal movement was established and analysed. For example, at 10% of stance phase, the stress concentration was observed on the lateral compartment due to the valgus moment created at heel strike. At the next interval, the stress concentration shifted to the medial side as the frontal knee moment shifted to a varus orientation. Discussion. The results suggest that the stress distribution of tibiofemoral articular cartilage is qualitatively consistent with the valgus and varus moment observed during the stance phase of gait. The methods described could be applied to investigate the effects of injury and reconstruction on stress distribution within the tibiofemoral joint

Objective: To examine the effect of varying the thickness of the cement mantle on the strain distribution near the bone-cement interface. Background: An insufficient cement mantle is thought to generate cement fractures near the bone-cement interface. Debonding at the bone-cement interface may accompany such fractures, and, mechanical failure of the prosthesis may follow. In this study, we aim to analyse the relationship between the cement mantle thickness and the acetabular strain distribution near the bone-cement interface. Experimental model: Four hemi-pelvic saw bones specimens were implanted with six protected precision strain gauges. All specimens were prepared to receive a 53/28 cemented polyethylene cup (Depuy Charnley Elite). Methods: We simulated hip joint force relative to the cup during normal walking for quasi-static tests on an Instron 1603 testing machine. The magnitude of the maximum and minimum principal strains, and the orientation of the maximum principal strains were calculated based on the readings of strains from a 32 channel digital acquisition system. Results: Statistically significant differences in the total strains per gait cycle (p< 0.001) have been noted at all gauge locations. In the principal load bearing quadrants, the recorded tensile strains are reduced by 50% as a result of the thicker mantle, while the transmission of compressive strain is enhanced. Conclusion: A cement mantle thickness of 5-6mm may preserve the structural integrity of the principal load bearing quadrants of the acetabulum better than a mantle thickness of 2-3mm, by minimising the acetabu-lar strains. This maybe desirable in total hip replacements for conditions such as rheumatoid arthritis and osteoporosis, where the poorer quality bone can be assisted by recruitment of a larger surface area to participate in load bearing. Keywords: Principal strains; Cement mantle; Mantle thickness; Bone-cement interface; Acetabular strains

Objective: To develop in-vitro experiments that measure the strain distributions at the bone-implant and bone-cement interface of the acetabular region under physiological loading conditions for cemented and cementless sockets. Experimental model: Four hemi-pelvic specimens of saw bones were used. Following careful placement of six protected precision strain gauges, two specimens were prepared to receive a cemented polyethylene cup (Depuy Charnley Elite 53/28). Another two specimens were prepared and implanted with un-cemented Duraloc 58/28 cups. Press-fit technique was validated by torque measurements. Background: Symptoms associated with prosthetic migration result from osteoclast induced bone resorption at the interface adjacent to bone. We aim to develop a new and more accurate method of measuring strains at this critical interface. Methods: To simulate quasi-static loading, selected variables of hip joint force relative to the cup during normal walking was used for quasi-static tests on an Instron 1603 testing machine. The magnitude and orientation of the principal strains (maximum and minimum) were calculated based on the readings of strains from a 32 channel digital acquisition system. Results: The magnitude and distribution of acetabular trabecular bone strains are dependent on the type of cup material (un-cemented/cemented) implanted. At the position of maximum load, the maximum principal strain in the un-cemented specimens was 14.4 times higher than that for the cemented specimens (T-value = −96.40, P-value = 0.007). The highest recorded tensile strains in these specimens were localised to the acetabular rim of the posterior-superior quadrant. For the cemented specimens, the maximum principal strains are highest in the dorsal acetabulum, at a location that approximates to the centre of rotation of the replaced hip joint. Shear strains in the posterior-superior quadrant of both cementless and cemented acetabuli surpass the maximum principal strains. Conclusion: In both cemented and un-cemented specimens, the maximum shear and principal strains magnitude show similar spatial and statistical distribution. As indicators of local failure prospect within the acetabulum, these strains suggest that the posterior-superior quadrant is the most likely site for load-induced micro-fractures, in both cemented and cementless acetabuli

Background: It is thought that the forces transmitted across the hip joint produce migration of the prosthesis by failure at either the bone-cement or the prosthesis-cement interface. As symptoms associated with such motions often result from failure at the cement-bone interface, it is this interface and its sub-surfaces that are the critical areas of prosthesis loosening. Our aim is to produce a new and more accurate method of measuring strains at this critical interface. Objective: To develop in-vitro experiments to measure the strain distributions near the bone-cement interface of the acetabulum region under physiological, quasi-static loading conditions. Experimental Model: Two hemi-pelvic specimens of saw bones were used. Following careful placement of six protected precision strain gauges (4.6 x 6.4mm, tri-axial EA-13-031RB-120/E). One specimen was prepared to receive a cemented polyethylene cup (Depuy Charnley Ogee LPW 53/22). An uncemented 58mm Duraloc cup was implanted into a second specimen. Methods: Hip joint force relative to the cup during normal walking (Bergmann, G., 2001. HIP98) was used for quasi-static tests on a Llody LR30K loading machine. The magnitude of the maximum and minimum principal strains, and the orientation of the maximum principal strains were calculated from a 32 channel digital acquisition system. Results: For both specimens, the maximum principal strains at the maximum loading were highest in the medial wall (dome area) of the acetabulum. The tensile strain from the dome of the uncemented specimen at the maximum loading was twice that of the cemented specimen. In the cemented specimen, the compressive strains in the medial wall were almost twice the tensile strains at the maximum load. Within the acetabular quadrants, the highest strains were recorded in the posterio-inferior quadrant. Compressive strains in the posterio-inferior wall of the acetabulum seem to be comparable to those in the anterior-superior wall. Conclusion: The critical areas for load transfer in the acetabulum are the medial wall (dome area), the posterio-inferior and the anterior-superior quadrants. The uncemented cup appears to provide a better load transfer mechanism than the cemented cup

Introduction. A new conservative hip stem has been designed to address the complex problem of total hip arthroplasty in the younger population. Objectives. To assess the stability and strain distribution of a new conservative hip stem. Materials and Methods. The prosthesis is tapered and collared and made from titanium (Ti6Al4V) with a titanium porous plasma spray to encourage bony ingrowth (Figure.1). It is circular-trapezoidal in cross-section to provide optimal ‘fit and fill’ in the femoral neck. (i) Finite Element Analysis (FEA). Computed tomography scans of an intact femur were modelled using MARC software and consisted of 161390 elements and 174881 nodes. The implant was modelled (Unigraphics) as a titanium alloy stem with a cobalt-chrome alloy head and consisted of 93440 hexahedral elements and 101133 nodes. This study compared the strains in the femoral calcar of an intact femur with a stem ‘implanted’ in neck shaft angles of 125°, 135°, and 145°. The head of all models received a load of 2.3KN at 7 degrees medially. (ii) Photoelastic Coating. A photoelastic coating was moulded around the medial cortices of ten third generation femora Sawbones. Strain before and after prosthesis insertion was measured at one-centimetre intervals down the medial cortex of the bones using a polariscope. The bones were positioned in a simplified single leg stance (7° physiological alignment), and loaded at 2.3 KN with strain recorded. (iii) Linear Variable Differential Transducers (LVDT's). Micromotion and migration of the prosthesis was measured using LVDT's. The femoral heads were cyclically loaded with 2.3KN at 1Hz for 2,500 cycles and held in a single leg stance. The bones were then repositioned at 70° of flexion to produce torsional (stair climbing) forces and loaded with 0.5KN for 2,500 cycles. Statistical analysis of non-parametric data was performed using a two-tailed Wilcox signed rank test (p<0.05). Results. The FEA analysis revealed strains in the neutral position most closely resembled that of an intact femur (Figure.2). Photoelastic strain readings for intact bone and following insertion were paired and statistically analysed using the Wilcox signed rank test (two tailed). The composite bones with prostheses inserted at 125° and 145° demonstrated a significant difference to the intact bones, whereas those at 135° showed no significant difference in the surface strain pattern of the femur following prosthetic insertion (Figure.3). Under single leg stance loading all prostheses produced axial micromotion of less than 200 µm and 50 µm in the varus-valgus direction. Implants inserted at 135° and 125° produced the least micromotion, the implants inserted at 145° had the greatest magnitude of motion and may be more susceptible to loosening. Under torsional load the same was true with the 135° and 125° producing the least micromotion while with the angulation of 145° micromotion increased over the test period – again suggesting loosening. Conclusion. This design transfers load in a physiological manner and the prosthesis is most stable in the neutral position. The findings from this study have been translated into clinical practice with the prosthesis implanted into two patients with promising results

Introduction. Traditional applied loading of the knee joint in experimental testing of RTKR components is usually confined to replicating the tibiofemoral joint alone. The second joint in the knee, the patellofemoral joint, can experience forces of up to 9.7 times body weight during normal daily living activities (Schindler and Scott 2011). It follows that with such high forces being transferred, particularly in high flexion situations such as stair climbing, it may be important to also represent the patellofemoral joint in all knee component testing. This research aimed to assess the inclusion of the patellofemoral joint during in vitro testing of RTKR components by comparing tibial strain distribution in two experimental rigs. The first rig included the traditional tibiofemoral joint loading design. The second rig incorporated a combination of both joints to more accurately replicate physiological loading. Five implanted tibia specimens were tested on both rigs following the application of strain gauge rosettes to provide cortical strain data through the bone as an indication of the load transfer pattern. This investigation aimed to highlight the importance of the applied loading technique for pre-clinical testing and research of knee replacement components to guide future design and improve patient outcomes. Methods. Five composite tibias (4th Generation Sawbones) were prepared with strain gauge rosettes (HBM), correctly aligned and potted using guides for repeatability across specimens. The tibias were then implanted with Stryker Triathlon components according to surgical protocol. The first experimental rig was developed to replicate traditional knee loading conditions through the tibiofemoral joint in isolation. The second experimental rig produced an innovative method of replicating a combination of the tibiofemoral and patellofemoral joint loading scenarios. Both rigs were used to assess the load distribution through the tibia using the same tibia specimens and test parameters for comparison integrity (Figure 1). The cortical strains were recorded under an equivalent 500 N cyclical load applied at 10° of flexion by a hydraulic test machine. Results. The average results comparing both experimental rigs at three strain gauge locations are shown in Figure 2. Paired t-tests were performed on all results and a p value of p<0.05 was considered significant. No significant differences were found between the rigs. There was a trend towards a reduction in proximal principal strain with the inclusion of the patellofemoral joint (p=0.058). Discussion. The results of this study indicate that there is no significant difference in tibial load transfer between the traditional and novel applied loading techniques at small flexion angles. There is a trend towards a reduction in proximal strain when including the patellofemoral joint. This reduction may be linked to the patella tendon force counteracting the effect of tibiofemoral loading at this small flexion angle. At high flexion angles the patellofemoral reaction load increases significantly relative to the tibiofemoral load. This will have a significant effect on tibial strains and so it is recommended that testing at higher flexion angles should be performed in a combined loading rig

In vitro loading of the proximal femur has improved our understanding of stress shielding after total hip arthroplasty. However, previous load simulators often use simplified loading regimens that may not produce physiologic baseline strains. The purpose of this study was to compare the femoral strain levels produced when using simplified and more complex loading.

A mechanical load simulator was developed which could simultaneously apply a spinal load and nine of eleven available muscle loads to the proximal femur in heelstrike and stair climbing modes. Computer controlled electromechanical actuators were attached to a strain gauged fresh cadaver femur (donor body weight 39 6kg) with metal cables. A spinal load of 668 N (SPL) was applied alone and in combination with individual muscle loads of 267 N to determine the effect of each muscle on femoral strain. The magnitude and direction of the joint reaction force (JRF) was monitored in real time by a three-dimensional force transducer proximal to a metal acetabulum. Anterior, middle and posterior portions of the gluteus medius (ABD), iliotibial band (ITB), short external rotators (SER), vastus lateralis, adductors, rectus femoris, hamstrings, iliopsoas, and gluteus maximus were simulated.

SPL was applied and ABD and ITB were adjusted to produce a JRF magnitude of 2.0 BW. SPL was applied with two combinations of nine muscle loads adjusted in heelstrike mode to produce a JRF magnitude of 2.0 and 2.5 BW and JRF trajectory aligned within one degree of the radiographically determined compression trabecular stream axis.

Both nine-muscle combinations produced lower medial compression strains and substantially lower lateral tension strains than SPL+ABD+ITB in heelstrike and stair climbing. Simplified loading caused a bending moment in the proximal femur resulting in higher strains. Combined loading at 2.5 BW produced compression at 10 of 12 gauges in heelstrike mode and 9 of 12 gauges in stairclimbing.

Chronic rotator cuff tears are a major problem especially in the elderly population. Refixation is associated with high re-rupture rates. Therefore new implants or healing methods are needed. For a control of success biomechanical characteristics of native as well as treated tendons are of particular importance. Currently, tensile tests with static material testing machines are the most common technique for the biomechanical characterization of tendons. Resulting values are the maximum force (Fmax), stiffness and the Young´s modulus. However, no information is given about the allocation of strains over the tendon area. In addition, the determination of Fmax results in tissue destruction thus foreclosing further evaluation like histology. The Digital Image Correlation (DIC) is a contact-free non-destructive optical measuring method which gives information about distribution of strains by tracking the areal shift of an applied speckle pattern. The needed speckle pattern has to have a high contrast, a homogeneous distribution and a good adhesion to the surface. The method is established for the characterization of construction materials [1] to detect e.g. weak points. The present study examined if DIC is applicable for the complementary biomechanical evaluation of the sheep infraspinatus tendon. Fine ground powder extracted from a printer cartridge was chosen as a starting point. Preliminary to the in vitro experiments, the powder was applied on sheets with different methods: brushing, blowing, sieving and stamping. Stamping showed best results and was used for further in vitro tests on cadaveric native tendons (n=5). First, the toner powder was transferred to coarse-grained abrasive paper using a brush and stamped on the tendon surface. Afterwards DIC analysis was performed. For the in vivo tests, the left infraspinatus tendon of two German black-headed Mutton Sheep was detached and then refixed with bone anchors, the right tendon was used as native control (authorization: AZ 33.19-42502-04-17/2739). 12 weeks after surgery the animals were euthanized, the shoulders were explanted and DIC measurement performed. The speckle pattern could be applied adequately on the smooth tendon surfaces of native tendons. All specimens could be analyzed by DIC with sufficient correlation coefficients. The highest displacements were measured in the peripheral areas, whereas the central part of the tendon showed a low displacement. Repaired left tendons showed obvious differences already macroscopically. The tendons were thicker and showed inhomogeneous surfaces. Application of the toner powder by stamping was distinctly more complicated, DIC analysis could not produce sufficient correlation coefficients. In summary, transfer of DIC to native infraspinatus tendons of sheep was successful and can be further transferred to other animal and human tendons. However, irregular surfaces in tendon scar tissues affect the application of an adequate speckle pattern with a stamp technique. Therefore, further modifications are necessary. This research project has been supported by the German Research Foundation “Graded Implants FOR 2180 – tendon- and bone junctions” WE 4262/6-1

Introduction. Vertebral compression fractures are the most common type of osteoporotic fracture. Though 89% of clinical fractures occur anteriorly, it is challenging to replicate these ex vivo with the underlying intervertebral discs (IVDs) present. Furthermore, the role of disc degeneration in this mechanism is poorly understood. Understanding how disc morphology alters vertebral strain distributions may lead to the utilisation of IVD metrics in fracture prediction, or inform surgical decision-making regarding instrumentation type and placement. Aim. To determine the effect of disc degeneration on the vertebral trabecular bone strain distributions in axial compression and flexion loading. Methods. Eight cadaveric thoracolumbar segments (T11-L3) were prepared (N=4 axial compression, N=4 flexion). µCT-based digital volume correlation was used to quantify trabecular strains. A bespoke loading device fixed specimens at the resultant displacement when loaded to 50N and 800N. Flexion was achieved by adding 6° wedges. Disc degeneration was quantified with Pfirrmann grading and T2 relaxation times. Results. Anterior axial strains were 80.9±39% higher than the posterior region in flexion (p<0.01), the ratio of which was correlated with T2 relaxation time (R. 2. =0.80, p<0.05). In flexion, the central-to-peripheral axial strain ratio in the endplate region was significantly higher when the underlying IVDs were non-degenerated relative to degenerated (+38.1±12%, p<0.05). No significant differences were observed in axial compression. Conclusion. Disc degeneration is a stronger determinant of the trabecular strain distribution when flexion is applied. Load transfer through non-degenerate IVDs under flexion appears to be more centralised, suggesting that disc degeneration predisposes flexion-type compression fractures by shifting high strains anteriorly. Conflicts of interest. The authors declare none. Sources of funding. This work was funded by the Engineering & Physical Sciences Research Council (EP/V029452/1), and Back-to-Back

Introduction and Objective. The patients with a total hip arthroplasty is growing in world manly in Europe and USA, and this solution present a high success at 10years in several orthopaedic registers. The application of total press-fit hip fixation presents the most used solution, but presents some failures associated to the acetabular component fixation, associated to the load transfer and bone loss at long term. The aim of this work is to investigate the influence of different acetabular bone loss in the strain distribution in iliac bone. To evaluate implant fixation, an experimental study was performed using acetabular press-fit component simulating different acetabular bone loss and measuring the strain distribution. Materials and Methods. The experimental samples developed was based in an iliac bone model of Sawbones supplier and a acetabular component Titanium (Stryker) in a condition press-fit fixation and was implanted according surgical procedure with 45º inclination angle and 20º in the anteversion angle. Were developed five models with same initial bone, one with intact condition simulating the cartilage between bones and four with different bone loss around the acetabular component. These four models representing the evolution of bone support of acetabular components presented in the literature. The evolution of bone loss was imposed with a CAD CAM process in same iliac bone model. The models were instrumented with 5 rosettes in critical region at the cortical bone to measure the strain evolution along the process. Results. The results of strain gauges present the influence of acetabular component implantation, reducing the bone strains and presented the effect of the strain shielding. The acetabular component works as a shield in the load transfer. The critical region is the posterior region with highest principal strains and the strain effect was observed with different bone loss around acetabular component. The maximum value of principal strain was observed in the intact condition in the anterior region, with 950μ∊. In the posterior superior region, the effect of bone loss is more important presenting a reduction of 500% in the strains. The effect of bone loss is presented in the strains induced with acetabular implantation, in the first step of implantation the maximum strain was 950μ∊ and in the last model the value was 50μ∊, indicating lower press-fit fixation. Conclusions. The models developed allows study the effect of bone loss and acetabular implant fixation in the load transfer at the hip articulation. The results presented a critical region as the anterior-superior and the effect of strain shielding was observed in comparison with intact articulation. The results of press-fit fixation present a reduction of implant stability along bone loss. The process of bone fixation developed present some limitation associated to the bone adhesion in the interface, not considered. Acknowledgement. This work was supported by POCI-01-0145-FEDER-032486,– FCT, by the FEDER, with COMPETE2020 - (POCI), FCT/M

Abstract. Introduction. Ankle arthritis is estimated to affect approximately 72 million people worldwide. Treatment options include fusion and total ankle replacement (TAR). Clinical performance of TAR is not as successful as other joint replacement and failure is poorly understood. Finite element analysis offers a method to assess the strain in bone implanted with a TAR. Higher strain has been associated with microfracture and alters the bone-implant interface. The aim of this study was to explore the influence of implant fixation on strain within the tibia when implanted with a TAR through subject-specific models. Methods. Five cadaveric ankles were scanned using a Scanco Xtreme CT. The Tibia and Talus were segmented from each scan and virtually implanted with a Zenith TAR (Corin, UK) according to published surgical technique. Patient specific models were created and run at five different positions of the gait cycle corresponding to peak load and flexion values identified from literature. Bone material properties were derived from CT greyscale values and all parts were meshed with linear tetrahedral elements. The implant-bone interface was adjusted to fully-fixed or frictionless contact, representing different levels of fixation post-surgery. Strain distributions around the tibial bone fixation were measured. Results. Initial results showed clear differences in strain distributions both between different ankle specimens and fixation levels, with highest strain occurring within the bone at the tip of the tibial stem. Frictionless contact gave higher strain outputs than fully-fixed for all specimens with a range 0.12–0.3% and 0.07%–0.13% respectively. Conclusions. In all specimens, strain was higher in the frictionless contact, which may be considered representative of no bony ingrowth, highlighting fixation may be a critical factor in TAR failure. Differences observed between specimens highlights that TAR may not be a suitable intervention for all patients, due to variation in bone quality and anatomy. Declaration of Interest. (b) declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported:I declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project

Background. In vivo evaluation of IVD strains is crucial to better understand normal and pathological IVD mechanics, and to evaluate the effectiveness of treatments. This study aimed to 1) develop a novel in vivo technique based on 3T MRI and digital volume correlation (DVC) to measure strains within IVDs and 2) to use this technique to resolve 3D strains within IVDs of healthy volunteers during extension. Methods. This study included 40 lumbar IVDs from eight healthy subjects. The optimal MR sequence to minimise DVC uncertainties was identified by scanning one subject with four different sequences: CISS, T1VIBE, T2SPACE, and T2TSE. To assess the repeatability of the strain measurements in spines with different anatomical and morphological variations four subjects were scanned with the optimal sequence, and uncertainties of the strain measurements were quantified. Additionally, to calculate 3D strains during extension, MRIs were acquired from six subjects in both the neutral position and after full extension. Results. Measurement errors were lowest when using the T2TSE sequence (precision=0.33 ± 0.10%, accuracy=0.48 ± 0.11%). The largest average maximum tensile and shear strains were seen at the L2-L3 level in all volunteers (7.2 ± 1.5% and 6.8 ± 1.1%, respectively), while the L5-S1 level experienced the lowest average tensile and shear strains (3.5 ± 1.0% and 3.9 ± 0.7%, respectively). Conclusion. The findings of this study establish clinical MRI-based DVC (MRI-DVC) as a new tool for in vivo strain measurement within human IVDs. MRI-DVC successfully provided internal strain distributions within IVDs and has great potential to be used for a wide range of clinical applications. Conflict of interest: No conflicts of interest. Source of funding: This work was supported by the EPSRC, New Investigator Award, EP/V029452/1

Introduction: Unicomparmental knee replacements have a long clinical history of success as well as failure. Recently, in Australia some 40% of knee surgery performed consists of unicompartmental knees for the treatment of medial compartment OA. This increased use of unicompartmental knees is in part due to advances in surgical technique through a minimally invasive approach. Loading conditions at the tibia-implant interface will play an important role in the stress/strain distributions at the proximal tibia. The use of an all PE tibial insert versus a metal backed component may provide a different strain disribution to the proximal tibia. This study examined the influence of metal backed and polyethylene tibial components in unicompartmental knee replacements with and without cement fixation on the initial strain distributions under various loading conditions. Materials and Methods: Three cadaveric tibias (mean age 47 years old) were cleaned of all soft tissue and strain gauged. Rosette strain gauges (TML Ltd., Tokyo, Japan) were placed at 2 levels on the tibial cortex. The intact tibia were embedded in a low melting point alloy at a standard height and tested using an MTS 858 Bionix testing machine (MTS Systems, Min., MI). The tibia were tested in nuetral, varus and valgus positions at zero and sixty degrees of flexion. A 1500N was applied for 15 seconds and the strains measured. A K-Scan sensor (Tekscan, Boston, MA) was used to confirm the varus and valgus loading positions and to obtain a contact footprint and pressure for the intact and reconstructed tibias under the loading conditions (Fig. 1). Following intact testing, the tibias were templated and reconstructed by a surgeon familiar with the technigue. The implants were investigated with and without cement fixation and compared to their respective all polyethylene component if it was available using the same loading regime as the intact tibias. Principal strains were calculated. Results: Tibial cortical strain distributions were significantly different at the proximal and distal sites under the loading conditions examined. The strain distribution for metal backed components was greater than the all PE design. Increasing flexion angle shifted the peak strains posteriorly. Metal backing and all PE tibial inserts presented different strain distributions on the medial side under nuetral and varus loading. Lateral compartment strains did not differ between designs, were higher proximal and decreased dramatically at the distal gauges. Cementless fixation tended to overload compared to the intact condition. Figure 2 presents the strain distribution for a typical metal backed and all poly unicompartmental knee in the nuetral position. Discussion: Metal backed unicompartmental components overloaded the proximal cortex of the tibia. All polyethylene tibial inserts did not overload the proximal cortex and had similar strain distribution to the intact tibia. Cemented fixation allows the transfer of load to the distal tibial cortex via the proximal cortex and subchondral bone, provided that the bone cement has inter-digitised the subchondral bone

Nuclear factor erythroid 2–related factor 2 (Nrf2) is a crucial transcription factor to maintain cellular redox homeostasis, but is also affecting bone metabolism. As the association between Nrf2 and osteoporosis in elderly females is not fully elucidated, our aim was to shed light on the potential contribution of Nrf2 to the development of age-dependent osteoporosis using a mouse model. Female wild-type (WT, n=18) and Nrf2-knockout (KO, n=12) mice were sacrificed at different ages (12 weeks=young mature adult, and 90 weeks=old), morphological cortical and trabecular properties of femoral bone analyzed by micro-computed tomography (µCT), and compared to histochemistry. Mechanical properties were derived from quasi-static compression tests and digital image correlation (DIC) used to analyze full-field strain distribution. Bone resorbing cells and aromatase expression by osteocytes were evaluated immunohistochemically and empty osteocyte lacunae counted in cortical bone. Wilcoxon rank sum test was used for data comparison and differences considered statistically significant at p<0.05. When compared to old WT mice, old Nrf2-KO mice revealed a significantly reduced trabecular bone mineral density (BMD), cortical thickness (Ct.Th), cortical area (Ct.Ar), and cortical bone fraction (Ct.Ar/Tt.Ar). Surprisingly, these parameters were not different in skeletally mature young adult mice. Metaphyseal trabeculae were thin but present in all old WT mice, while no trabecular bone was detectable in 60% of old KO mice. Occurrence of empty osteocyte lacunae did not differ between both groups, but a significantly higher number of osteoclast-like cells and fewer aromatase-positive osteocytes were found in old KO mice. Furthermore, female Nrf2-KO mice showed an age-dependently reduced fracture resilience when compared to age-matched WT mice. Our results confirmed lower bone quantity and quality as well as an increased number of bone resorbing cells in old female Nrf2-KO mice. Additionally, aromatase expression in osteocytes of old Nrf2-KO mice was compromised, which may indicate a chronic lack of estrogen in bones of old Nrf2-deficient mice. Thus, chronic Nrf2 loss seems to contribute to age-dependent progression of female osteoporosis

Introduction. Revision total knee arthroplasy (TKA) has been often used with a metal block augmentation for patients with poor bone quality. However, bone resorption beneath metal block augmentation has been still reported and little information about the reasons of the occurrence of bone resorption is available. The aim of the current study is to identify a possibility of the potential occurrence of bone resorption beneath metal block augmentation, through evaluation of strain distribution beneath metal block augmentation in revision TKA with metal block augmentation, during high deep flexion. Materials and Method. LOSPA, revision TKA with a metal block augmentation (Baseplate size #5, Spacer size #5, Stem size Φ9, L30, Augment #5 T5) was considered in this study. For the test, the tibia component of LOSPA was implanted to the tibia sawbone (left, #3401, Sawbones EuropeAB, Malmö, Sweden), which was corresponded to a traditional TKR surgical guideline. The femoral component of LOSPA was mounted to a customized jig attached to the Instron 8872 (Instron, Norwood, MA, USA), which was designed specially to represent the angles ranged from 0° to 140° with consideration of a rollback of knee joint (Figure. 1). Here, a compressive load of 1,600N (10N/s) was applied for each angle. Strain distribution was then measured from rossete strain gauge (Half Bridge type, CAS, Seoul, Korea) together (Figure 1). Results and Discussions. The strain distribution on the cortical bone of the tibia was shown in Figure 2. The results showed that the strains on the posterior region were gradually increased from extension to high deep of the knee joint and generally larger than the other regions. In contrast to the results on the posterior region, the strains on the medial region were gradually decreased after 60° or 90° flexion position and relatively lower than the other regions. Particularly, the strains on the medial region were generally lower than 50–100 µstrain, which is known as critical value range able to inducing bone resorption, during high deep flexion. This fact indicate that a possibility of the potential bone resorption occurrence in revision TKA used with a metal block augmentation may be relatively increased in patients who are frequently exposed to a personal lifestyle history with the loading conditions of the high flexion. This study may be valuable by identifying for the first time a possibility of the potential bone resorption occurrence through evaluation of the strain distribution beneath metal block augmentation in revision TKA used with a metal block augmentation during high deep flexion. Conclusion. A possibility of the potential bone resorption occurrence in revision TKA used with a metal block augmentation may be dependent on loading patterns applied on the knee joint related to personal lifestyle history. Particularly, it may be relatively increased in patients who are frequently exposed to a personal lifestyle history with the loading conditions of the high flexion. Acknowledgements. This study was supported by a grant from the New Technology Product Evaluation Technical Research project, Ministry of Food and Drug Safety (MFDS), Republic of Korea

Background. Trabecular metal (TM) cones are designed to fill up major bone defects in total knee arthroplasty. Tibial components can be implanted in combination with a stem, but it is unclear if this is necessary after reconstruction with a TM cone. Implanting a stem may give extra stability, but may also have negative side-effects. Aim of this study was to investigate stability and strain distribution of a tibial plateau reconstruction with a TM cone while the tibal component is implanted with and without a stem, and whether prosthetic stability was influenced by bone mineral density (BMD). Methods. Tibial revision arthroplasties were performed after reconstruction of an AORI 2B bone defect with TM cones. Plateaus were implanted in seven pairs of cadaveric tibiae; of each pair, one was implanted with and the other without stem. All specimens were loaded to one bodyweight alternating between the medial and lateral tibia plateau. Implant-bone micro motions, bone strains, BMD and correlations were measured and/or calculated. Results. Tibial components without a stem showed only more varus tilt (difference in median 0.14 degrees (P<0.05), but this was not considered clinically relevant. Strain distribution did not differ. BMD had only an effect on the anterior/posterior tilt ρ:-0.72 (P<0.01). Conclusions. Tibial components, with or without a stem, which are implanted after reconstruction of major bone defects using TM cones produce very similar biomechanical conditions in terms of stability and strain distribution. Additional stem extension of the tibial component may not be required after reconstruction of major bone defects using TM cones. Level of evidence. IIb. Disclosures. The department of Orthopaedics, University of Groningen, University Medical Center Groningen has received direct funding from the Anna Fonds (Oegstgeest, NL). Zimmer (Warsaw, IN, USA) has provided the instrumentation and tools for this study. The department of Orthopaedics, University of Groningen, University Medical Center Groningen receives research institutional support from InSpine (Schiedam, NL) and Stryker (Kalamazoo, Mich. USA). One of the authors (ALB) will be and has been paid as a consultant by Zimmer (Warsaw, IN, USA) for purposes of education and training in knee arthroplasty