Aim

This study aims to evaluate the effectiveness of a pre-formulated irrigation solution¹ (containing ethanol, acetic acid, sodium acetate, benzalkonium chloride, and sterile water) compared to saline solution in managing acute periprosthetic joint infections (A-PJI) during Debridement, Antibiotic, and Implant Retention (DAIR) surgeries. The primary objective is to assess the healing rate using this solution¹ versus saline in A-PJI patients, with “cure” defined by a set of criteria including no recurrence, wound issues, or need for ongoing suppressive antibiotics after 1 year. Principio del formularioFinal del formulario

Aim

External fixator knee arthrodesis is a salvage procedure mainly used in cases of end-stage infected total knee replacement (iTKR). A stable fixation combined with bone-ends compression is basic to achieve knee fusion in such a scenario but providing enough stability can be challenging in the presence of severe bone loss after multiple previous procedures. Compared with monoplanar configuration, a biplanar frame achieves improved coronal stiffness, while providing the advantages of good access to the wound and allowance of early ambulation. Our primary hypothesis stated that a biplanar frame would achieve higher and quicker fusion rate than a monolateral configuration.

Aim

The ultimate diagnostic proof of chronic osteomyelitis (COM) is the association of a compatible clinical presentation with an unequivocal positive deep bone sample culture. Intraoperative deep bone samples cultures has been widely considered the gold standard in this setting but the preoperative identification of the infecting microorganism through a bone biopsy is of paramount importance in the diagnostic and treatment protocol of any COM. Unfortunately, preoperative bone biopsies have proven to have a broad range of sensitivity values and the most useful biopsy technique remains unknown. The correlation of the preoperative and intraoperative microbiological results is a matter of concern. The purpose of this study was to assess the diagnostic accuracy of a percutaneous bone biopsy (PBB) and an open bone biopsy (OBB) in isolating the infecting organism in cases of lower extremity chronic osteomyelitis.

Ankle osteomyelitis after open pilon-fractures remain one of the most challenging scenarios. Ankle-fusion using an external frame is a classical option but in some cases of non-compliance patients could be not ideal. The purpose of this report was to evaluate our results using a new staged salvage protocol in cases where an external-frame arthrodesis is not recommended due to the issue of a non-compliance patient.

During the first stage we resect all the infected tissues and an antibiotic-loaded cement spacer is used to obliterate the dead space, to reach some kind of stability and to achieve a high concentration of local antibiotic. A free or keystone flap is used if needed and a posterior splint is placed. After a course of targeted systemic antibiotics the second stage is schedule. During the second stage after the spacer removal, a self-made antibiotic coating retrograde hindfoot arthrodesis nail is used in order to achieve ankle fusion. We use a bone-substitute loaded with gentamicin (Cerament-G®) to fill the bone defects and to achieve high concentration of local gentamicin, to protect bone healing and to decrease the risk of relapse.

We present four patients with ankle-osteomyelitis after open pilon fracture treated by such protocol. We receive all patients > 6 months after the initial fracture. In all cases type III-B open pilon fracture was the initial injury. All the patients presented psychological disturbances and the possibility of perform an external frame reconstruction was considered not indicated. At presentation, all patients have compromised skin around the distal tibia area. According to the Cierny/Mader osteomyelitis classification all patients were Type IV-Blocal. Compromises soft tissues were treated with an ALT free flap in two cases and with a Keystone flap in two cases. 10 mL paste of Cerament-G® was used in each case with a single case where a white-draining event was present without further complication. Tibiotalocalcaneal fusion was achieved after 4 months in all cases. At average of 7 months of follow-up, none of the four fused ankles has required further surgery and no infection relapse was observed.

The use of this salvage treatment protocol has proven safe and useful for such difficult problem in these difficult patients, with a relatively low associated complication rate. Cerament-G® is a useful tool in order to fill all the bone voids, promote bone healing and simultaneously to protect the surgical area due to the high local concentration of gentamicin.

Necrotising fasciitis (NF) of the extremities is a rapidly progressive, potentially life threatening soft tissue infection. Recent advances in its management, like hidrobisturi-assisted debridement (Versajet®), negative pressure wound therapy (NPWT), or Intravenous Immunoglobulin (IVIG) have not clearly influenced in mortality and morbidity rates, still high. We therefore sought to study the necrotising fasciitis of the extremities diagnosed in the last four year in our hospital. We investigate (1) the morbidity and mortality rates, (2) the microbiologic characteristics of the infection, and (4) the management focusing on the use of new treatment technologies.

This is a 4-year retrospective chart review of all NF of the extremities who presented in our hospital, from 2010 through 2013. We collected data on demographics, comorbidities, diagnostic test, LRINEC score and microbiological information. We evaluated the therapeutic management of these patients, focusing in the intensive care necessities, the use of hidrobisturi and NPWT as well the treatment with IVIG

20 patients satisfied our inclusion criteria. Lower extremity was the most common location of infection (60%). Blood cultures were available in 14 cases, 7 with a negative culture result (50%). The average LRINEC score on the day of presentation was 6 (range: 0–11). All the patients were treated operatively with 2.5 interventions on average (range: 1 to 5 operations). In the operative samples, one or more causative microorganisms were identified in 18/20 (90%) of the NF cases, with two culture negative cases. Overall, type II NF (Group A β-haemolytic streptococci) was found in 11 cases (55%) and Type I (synergistic polymicrobial) in 7 cases (35%). Versajet® was used in the first debridement in 40% (8 out 20) of the cases and in the second-look in 80% of the cases. In 5 cases (25%) a direct wound closure was selected and in 75% cases a VAC closure was the technique of choice. Thirteen patients (65%) were admitted in the intensive care unit, with a medium stay of 12 days. The overall mortality was 30 % with LRINEC score, glucose level and creatinin level being an independent risk factor of death (p < .05). Five amputations were identified in this series (25%).

According our data, despite surgical advances, pharmacological new drugs and intensive care improvements, NF remains a disease with high mortality and morbidity. New technologies have been used widely in the last four years in our center without appearing to influence the final outcome of the disease.

Acute postoperative periprosthetic joint infection (PJI) is a serious complication after any hemiarthroplasty (HHA) implanted due to a proximal hip fracture. The growing number of chronic institutionalized geriatric patients (CIGP) colonized with multi-drug resistant bacteria (e.g.: MRSA), not covered by usual antibiotic prophylaxis, has been identified as a risk factor for PJI after HHA. We therefore sought to compare the HHA infection characteristics between non-institutionalized patients (NIP) with proximal hip fractures and CIGP. We investigate (1) the rate of compliance with a new proposed protocol, (2) the acute infection rate, 3) the microbiologic characteristics of the infection, and 4) the success of the new protocol.

We gathered clinical, operative and infection data on all patients who underwent HHA due to a proximal femoral fracture in our center, during a 3-year period. We focus in the cases of acute postoperative infection (Zimmerli´s criteria). The new proposed antibiotic prophylaxis is cefazolin except in CIGP in which co-trimoxazole is used.

During the study period a total of 385 HHA in 385 patients were performed. In all cases the HHA was performed after a proximal femoral fracture. Overall, 109 patients (28,2%) were CIGP. We found an acute postoperative PJI in 21 out 385 HHA procedures, that is, a global acute infection rate of 5.43%. Ten out 109 (9.17%) CIGP patients resulted infected compared to 11 out 278 (3.9%) non-institutionalized patients (p: 0.049). One or more causative microorganisms were identified in 20/21 (95%) of PJI. Globally the Gram-Negative bacilli group accounted for the majority of the infections (60%). Staphylococus aureus was isolated in 3 cases (8.6%) with only a single MRSA infection. The percentage of polymicrobial infections was 47% (10 out of 21). Co-trimoxazole was used in the prophylaxis in 80.1% of the CIGP. In the infected cases a non-effective drug against the microorganism was used in the prophylaxis in 17 (81%) of the acute infected HHA.

We confirm that institutionalized patients are more prone to acute infections after a HHA. Our current strategy of antibiotic prophylaxis has showed to be effective in preventing MRSA PJI in CIGP. However, we found an increased rate of infection due to gram-negative bacilli non-covered by the current antibiotic prophylaxis. According our data an extended antibiotic prophylaxis on gram-negative drug will be proposed to be implemented in CIGP scheduled to a HHA because a proximal femoral fracture.

Aims

Currently, the most common approach for the management of a chronic PJI is a Two-Stage Replacement; because of success rates exceeding 90% when using an antibiotic impregnated cement spacer. Reliable information regarding the etiologic microorganism and its sensitivities is essential to select the antimicrobial therapy that should be used locally in the bone cement spacer during the first stage surgery as well as to select the appropriate microbiological systemic agent. Diagnostic algorithms focus to the importance of joint aspiration cultures although in the modern literature, preoperative joint aspiration has a broad range of values of sensitivity and the proportion of “dry-aspirations” is not well assessed. This low sensitivity of aspiration fluid samples in chronic-PJI is partly attributable to the fact that the majority of the microorganisms in these infections grow in biofilms attached to the implant. We have developed this biopsy technique in an effort to improve the identification rates of the causative organism.

Amniotic band syndrome (ABS) is a congenital disorder characterized by limb constrictions. The disorder lacks precise definition, and its exact pathogenesis is unknown. Though theories have been advanced to explain the condition’s origin, none have been scientifically validated. The “exogenous” theory, popularized by Torpin, is the most widely accepted. It suggests that early amniotic rupture leads to formation of amniotic strands, which by means of progressive compression induce formation of extremity bands. In this disorder, histological examination of the excised bands demonstrates them to be composed of fibrous tissue. Multiple clinical and experimental data reveal this theory has only low plausibility. Our purpose is to assess whether annular external compression of a fetal rabbit limb will produce a band of subcutaneous fibrous tissue characteristic of amniotic band syndrome.

We operated on one limb of 10 different rabbit fetuses, each at 21 days of gestation. The extremity was ligated with a nylon suture at the infracondylar level. At 30 days gestation, each fetus was delivered by caesarean section. Limbs were analyzed histologically using different techniques. Histological analysis did not show subcutaneous fibrous tissue in the mechanically constricted zone. The distal segment showed dilatation of lymphatic vessels and edema of soft tissue.

Annular external compression of a fetal rabbit limb does not induce development of new fibrous tissue; therefore this experimental study does not support the theory of a mechanical exogenous pathogenesis in amniotic band syndrome.

Introduction. With the use of fetoscopy (minimally invasive surgery), the indications for foetal surgery have been expanded. Extremities at risk of amputation by EAB are amenable to foetal surgery. A previous model of in uterus reparation of moderate EAB has been reported. The purpose of this study is to evaluate the feasibility of intrauterine foetal release of amputating EAB.

Material and methods. Right limbs of fifteen 60-days gestational age pregnant sheep were ligated with silk suture at the infracondilar level. Left limbs were used for paired comparison. Foetuses were randomized in three groups: early repair group (n=5), late repair group (n=5) and no-repair group (n=5). The limbs of repaired groups underwent foetal release. The limbs obtained from at term foetuses were analyzed morphologically, functionally, radiologically, and histologically. Statistical analysis with paired test was used to compare data.

Results. Non-repaired limbs showed amputation or quasi-amputation; the repaired ones did not. However, those late repaired had significantly reduced passive ankle range of motion, shorter limb length, and mild residual changes.

Conclusions. In uterus release of potentially severe EAB avoids limb amputation and permits its morphological and functional recovery. Early release shows better results.