The incidences of fragility fractures, often because of osteoporosis, are increasing. Research has moved towards bioresorbable scaffolds that provide temporary mechanical stability and promote osteogenesis. This research aims to fabricate a 3D printed composite Poly (l-lactic-co-glycolic acid)-strontium doped tricalcium phosphate (PLGA-SrTCP) scaffold and evaluate in an in vitro co culture study containing osteoporotic donor cells. PLGA, PLGA TCP, and PLGA SrTCP scaffolds were produced using Fused Filament Fabrication (FFF). A four-group 35-day cell culture study was carried out using human bone marrow derived mesenchymal stem cells (hMSCs) from osteoporotic and control donors (monoculture) and hMSCs & human monocytes (hMCs) (Co culture). Outcome measures were biochemical assays, PCR, and cell imaging. Cells were cultured on scaffolds that had been pre-degraded for six weeks at 47°C prior to drying and gamma sterilisation.Introduction
Method
Osteoporotic fractures tend to be more challenging than fractures in healthy bone and the efficacy of metal screw fixation decreases with decreasing bone mineral density making it more difficult for such screws to gain purchase. This leads to increased complication rates such as malunion, non-union and implant failure (1). Bioresorbable polymer devices have seen clinical success in fracture fixation and are a promising alternative for metallic devices but are rarely used in the osteoporotic population. To address this, we are developing a system that may allow osteoporotic patients to avail of bioresorbable devices (2) but it is important to establish if patients have any reservations about having a plastic resorbable device instead of a metal one. Therefore the aim of this study was to explore the acceptability of bioresorbable fracture fixation devices to people with osteoporosis. A cross sectional descriptive study was conducted in a UK wide population using convenience sampling. An online survey comprising nine survey questions and nine demographic questions was developed in Microsoft Teams and tested for face validity in a small pilot study (n=6). Following amendments and ethical approval, the survey was distributed by the Royal Osteoporosis Society on their website and social media platforms. People were invited to take part if they lived in the UK, were over 18 years old and had been diagnosed with osteoporosis. The survey was open for three weeks in May 2023. Responses were analysed using descriptive statistics.Abstract
Objectives
Methods
Current strategies for bone repair have accepted limitations and the search for synthetic graft materials or for scaffolds that will support ex vivo bone tissue engineering continues. Bioprospecting has led to increased interest in potential applications for marine organisms and their by-products and biomimetic strategies have led to the investigation of naturally occurring porous structures as templates for bone growth. As a rich source of mineralising porous organisms, our seas and oceans could provide new directions for bone tissue engineering that may enhance
Metal on metal press-fit acetabular cups are the worst performing acetabular cup type with severe failure consequences compared to cups made from more inert materials such as polyethylene or ceramic. The cause of failure of these cup types is widely acknowledged to be multi-factorial, therefore creating a complex scenario for analysis through clinical studies. A factorial analysis has been carried out using an experimentally validated finite element analysis to investigate the relative influence of four input factors associated with acetabular cup implantation on output parameters indicating potential failure of the implantation. These input factors were: cup material stiffness; cup inclination; cup version; cup seating; and level of press-fit. The output parameter failure indicators were: wear; tensile strains in the underlying bone; bone remodelling; and cup-bone micromotions. The factorial analysis concluded that the most significant influence was that of cup inclination on wear, and the second most significant was the influence of the level of press-fit on bone remodelling at the acetabular rim. Significant influence was also observed between version angle and wear, and cup-seating and micro-motion. The results demonstrated the clear multi-factorial nature of implant failure and highlighted the importance of correct implant positioning and fit.
