Mesenchymal stem cells (MSCs) are usually cultured in a normoxic atmosphere (21%) in vitro, while the oxygen concentrations in human tissues and organs are 1% to 10% when the cells are transplanted in vivo. However, the impact of hypoxia on MSCs has not been deeply studied, especially its translational application. In the present study, we investigated the characterizations of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in hypoxic (1%) and normoxic (21%) atmospheres with a long-term culture from primary to 30 generations, respectively. The comparison between both atmospheres systematically analyzed the biological functions of MSCs, mainly including stemness maintenance, immune regulation, and resistance to chondrocyte apoptosis, and studied their joint function and anti-inflammatory effects in osteoarthritis (OA) rats constructed by collagenase II.Aims
Methods
To investigate the risk factors for unsuccessful radial head reduction (RHR) in children with chronic Monteggia fractures (CMFs) treated surgically. A total of 209 children (mean age 6.84 years (SD 2.87)), who underwent surgical treatment for CMFs between March 2015 and March 2023 at six institutions, were retrospectively reviewed. Assessed risk factors included age, sex, laterality, dislocation direction and distance, preoperative proximal radial metaphysis width, time from injury to surgery, reduction method, annular ligament reconstruction, radiocapitellar joint fixation, ulnar osteotomy, site of ulnar osteotomy, preoperative and postoperative ulnar angulation, ulnar fixation method, progressive ulnar distraction, and postoperative cast immobilization. Independent-samples Aims
Methods
To determine the risk of total knee replacement (TKR) for primary osteoarthritis (OA) associated with overweight/obesity in the Australian population. This population-based study analyzed 191,723 cases of TKR collected by the Australian Orthopaedic Association National Joint Registry and population data from the Australian Bureau of Statistics. The time-trend change in incidence of TKR relating to BMI was assessed between 2015-2018. The influence of obesity on the incidence of TKR in different age and gender groups was determined. The population attributable fraction (PAF) was then calculated to estimate the effect of obesity reduction on TKR incidence. The greatest increase in incidence of TKR was seen in patients from obese class III. The incidence rate ratio for having a TKR for obesity class III was 28.683 at those aged 18-54 years but was 2.029 at those aged >75 years. Females in obesity class III were 1.7 times more likely to undergo TKR compared to similarly classified males. The PAFs of TKR associated with overweight or obesity was 35%, estimating 12,156 cases of TKR attributable to obesity in 2018. The proportion of TKRs could be reduced by 20% if overweight and obese population move down one category. Obesity has resulted in a significant increase in the incidence of TKR in the youngest population in Australia. The impact of obesity is greatest in the young and the female population. Effective strategies to reduce the national obese population could potentially reduce 35% of the TKR, with over 10,000 cases being avoided.
Morphological abnormalities are present in patients with developmental dysplasia of the hip (DDH). We studied and compared the pelvic anatomy and morphology between the affected hemipelvis with the unaffected side in patients with unilateral Crowe type IV DDH using 3D imaging and analysis. A total of 20 patients with unilateral Crowe-IV DDH were included in the study. The contralateral side was considered normal in all patients. A coordinate system based on the sacral base (SB) in a reconstructed pelvic model was established. The pelvic orientations (tilt, rotation, and obliquity) of the affected side were assessed by establishing a virtual anterior pelvic plane (APP). The bilateral coordinates of the anterior superior iliac spine (ASIS) and the centres of hip rotation were established, and parameters concerning size and volume were compared for both sides of the pelvis.Aims
Methods
Vancouver type B periprosthetic femoral fractures (PFF) are challenging complications after total hip arthroplasty (THA), and some treatment controversies remain. The objectives of this study were: to evaluate the short-to-mid-term clinical outcomes after treatment of Vancouver type B PFF and to compare postoperative outcome in subgroups according to classifications and treatments; to report the clinical outcomes after conservative treatment; and to identify risk factors for postoperative complications in Vancouver type B PFF. A total of 97 consecutive PPFs (49 males and 48 females) were included with a mean age of 66 years (standard deviation (SD) 14.9). Of these, 86 patients were treated with surgery and 11 were treated conservatively. All living patients had a minimum two-year follow-up. Patient demographics details, fracture healing, functional scores, and complications were assessed. Clinical outcomes between internal fixation and revisions in patients with or without a stable femoral component were compared. Conservatively treated PPFs were evaluated in terms of mortality and healing status. A logistic regression analysis was performed to identify risk factors for complications.Aims
Methods
Only a small number of studies exist that report the results of EBM-produced porous coated trabecular titanium cups in primary total hip arthroplasty (THA). This study aims to investigate the patient satisfaction level, clinical function and radiographic outcomes of the patients who underwent THA using an EBM-produced porous coated titanium cup. A total of 32 patients who underwent primary THA with using an EBM-produced porous coated titanium cup from five hospitals between May and December, 2012 were retrospectively reviewed. Five patients were lost prior to the minimum 6-year follow-up. Clinical and radiographic outcomes were analyzed with an average follow-up of 81.48 (range: 77.00–87.00) months.Aims
Patients and Methods
The incidence of thigh pain with the short stem varies widely across different studies. We aimed to evaluate the incidence and characteristics of post-operative thigh pain after using a particular bladed short stem and its potential risk factors. We respectively reviewed 199 consecutive patients who underwent unilateral total hip replacement using the Tri-lock stem from 2013–2016, of which 168 patients were successfully followed up with minimum two year clinical follow-up. All information about thigh pain and pre- and postoperative HHS score were gathered and all preoperative and immediate postoperative radiographs were available for review. Any complications were recorded.Aims
Patients and Methods
Failed ingrowth and subsequent separation of revision acetabular components from the inferior hemi-pelvis constitutes a primary mode of failure in revision total hip arthroplasty (THA). Few studies have highlighted other techniques than multiple screws and an ischial flange or hook of cages to reinforce the ischiopubic fixation of the acetabular components, nor did any authors report the use of porous metal augments in the ischium and/or pubis to reinforce ischiopubic fixation of the acetabular cup. The aims of this study were to introduce the concept of extended ischiopubic fixation into the ischium and/or pubis during revision total hip arthroplasty [Fig. 2], and to determine the early clinical outcomes and the radiographic outcomes of hips revised with inferior extended fixation. Patients who underwent revision THA utilizing the surgical technique of extended ischiopubic fixation with porous metal augments secured in the ischium and/or pubis in a single institution from 2014 to 2016 were reviewed. 16 patients were included based on the criteria of minimum 24 months clinical and radiographic follow-up. No patients were lost to follow-up. The median duration of follow-up for the overall population was 37.43 months. The patients' clinical results were assessed using the Harris Hip Score (HHS), Western Ontario and McMaster Universities Osteoarthritis (WOMAC) index and Short form (SF)-12 score and satisfaction level based on a scale with five levels at each office visit. All inpatient and outpatient records were examined for complications, including infection, intraoperative fracture, dislocation, postoperative nerve palsy, hematoma, wound complication and/or any subsequent reoperation(s). The vertical and horizontal distances of the center of rotation to the anatomic femoral head and the inclination and anteversion angle of the cup were measured on the preoperative and postoperative radiographs. All the postoperative plain radiographs were reviewed to assess the stability of the components.Background
Methods
Severe, superior acetabular bone defects are one of the most challenging aspects to revision total hip arthroplasty (THA). We propose a new concept of “superior extended fixation” as fixation extending superiorly 2 cm beyond the original acetabulum rim with porous metal augments, which is further classified into intracavitary and extracavitary fixation. We hypothesized that this new concept would improve the radiographic and clinical outcomes in patients with massive superior acetabular bone defects. Twenty eight revision THA patients were retrospectively reviewed who underwent reconstruction with the concept of superior extended fixation from 2014 to 2016 in our hospital. Patients were assessed using the Harris Hip Score (HHS) and the Western Ontario and McMaster Universities Osteoarthritis Index score (WOMAC). In addition, radiographs were assessed and patient reported satisfaction was collected.Aims
Patients and Methods
Injections of hyaluronic acid solutions, often known as visco-supplements, into the joints of patients suffering from osteoarthritis are an accepted therapy. In most replacement joints, wear of the biomaterials used in them is a critical concern. For in vitro wear tests of such materials the recommended lubricant is one based on bovine serum. However, unlike synovial fluid, bovine serum does not contain hyaluronic acid. The aim of the work reported here was to take a clinically used hyaluronic acid solution, Ostenil, and to investigate its influence on the wear of two orthopaedic biopolymers Ultra high molecular weight polyethylene (UHMWPE) and poly tetra fluoro ethylene (PTFE) were tested in turn using a four-station, multi-directional, pin-on-plate wear test rig which had previously been shown to reproduce clinical wear factors for UHMWPE, PTFE and polyacetal. For each biopolymer three lubricants were employed: 33% bovine serum (2 stations); 33% bovine serum + Ostenil (1 station); and distilled water + Ostenil (1 station). Polymeric test pins were subject to a load of 40N and articulated against polished stainless steel plates. Wear factors were determined by dividing the volume lost by the product of the load and the sliding distance (units × 10-6mm3/Nm). The UHMWPE wear tests ran to 66.3km sliding distance. The addition of Ostenil to dilute bovine serum resulted in a wear factor of 1.4 × 10-6mm3/Nm for UHMWPE. The wear factor was 1.6 × 10-6mm3/Nm when dilute bovine serum alone was used as the lubricant. This shows good agreement with a wear factor of 2.1 × 10-6mm3/Nm reported for failed UHMWPE acetabular cups. PTFE provides an accelerated wear test with clinical validity. In the presence of 33% bovine serum a mean wear factor for PTFE of 40 × 10-6mm3/Nm was measured. The wear factor was 59 × 10-6mm3/Nm for dilute bovine serum plus Ostenil. For explanted PTFE acetabular cups a wear factor of 37 × 10-6mm3/Nm has been calculated. For both polymers wear was least when the lubricant was distilled water plus Ostenil. However a transfer film was found and such films are not clinically valid.
We operated on 111 patients with 159 congenital club feet with the aim of correcting the deformity and achieving dynamic muscle balance. Clinical and biomechanical assessment was undertaken at least six years after operation when the patient was more than 13 years of age. The mean follow-up was for 11 years 10 months (6 to 36 years). Good and excellent results were obtained in 91.8%. Patients with normal function of the calf had a better outcome than those with weak calf muscles. The radiological changes were assessed in relation to the clinical outcome. The distribution of pressure under the foot was measured for biomechanical assessment. Our results support the view that muscle imbalance is an aetiological factor in club foot. Early surgery seems to be preferable. It is suggested that operation should be undertaken as soon as possible after the age of six months, although it may be carried out up to the age of five years. The establishment of dynamic muscle balance appears to be an effective method of maintaining correction. Satisfactory long-term results can be achieved with adequate appearance and function.
