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Bone & Joint Research
Vol. 13, Issue 7 | Pages 362 - 371
17 Jul 2024
Chang H Liu L Zhang Q Xu G Wang J Chen P Li C Guo X Yang Z Zhang F

Aims

The metabolic variations between the cartilage of osteoarthritis (OA) and Kashin-Beck disease (KBD) remain largely unknown. Our study aimed to address this by conducting a comparative analysis of the metabolic profiles present in the cartilage of KBD and OA.

Methods

Cartilage samples from patients with KBD (n = 10) and patients with OA (n = 10) were collected during total knee arthroplasty surgery. An untargeted metabolomics approach using liquid chromatography coupled with mass spectrometry (LC-MS) was conducted to investigate the metabolomics profiles of KBD and OA. LC-MS raw data files were converted into mzXML format and then processed by the XCMS, CAMERA, and metaX toolbox implemented with R software. The online Kyoto Encyclopedia of Genes and Genomes (KEGG) database was used to annotate the metabolites by matching the exact molecular mass data of samples with those from the database.


Bone & Joint Research
Vol. 12, Issue 9 | Pages 546 - 558
12 Sep 2023
Shen J Wei Z Wang S Wang X Lin W Liu L Wang G

Aims

This study aimed to evaluate the effectiveness of the induced membrane technique for treating infected bone defects, and to explore the factors that might affect patient outcomes.

Methods

A comprehensive search was performed in PubMed, Embase, and the Cochrane Central Register of Controlled Trials databases between 1 January 2000 and 31 October 2021. Studies with a minimum sample size of five patients with infected bone defects treated with the induced membrane technique were included. Factors associated with nonunion, infection recurrence, and additional procedures were identified using logistic regression analysis on individual patient data.


Bone & Joint Research
Vol. 11, Issue 7 | Pages 426 - 438
20 Jul 2022
Luo P Wang P Xu J Hou W Xu P Xu K Liu L

Rheumatoid arthritis (RA) is an autoimmune disease that involves T and B cells and their reciprocal immune interactions with proinflammatory cytokines. T cells, an essential part of the immune system, play an important role in RA. T helper 1 (Th1) cells induce interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α), and interleukin (IL)-2, which are proinflammatory cytokines, leading to cartilage destruction and bone erosion. Th2 cells primarily secrete IL-4, IL-5, and IL-13, which exert anti-inflammatory and anti-osteoclastogenic effects in inflammatory arthritis models. IL-22 secreted by Th17 cells promotes the proliferation of synovial fibroblasts through induction of the chemokine C-C chemokine ligand 2 (CCL2). T follicular helper (Tfh) cells produce IL-21, which is key for B cell stimulation by the C-X-C chemokine receptor 5 (CXCR5) and coexpression with programmed cell death-1 (PD-1) and/or inducible T cell costimulator (ICOS). PD-1 inhibits T cell proliferation and cytokine production. In addition, there are many immunomodulatory agents that promote or inhibit the immunomodulatory role of T helper cells in RA to alleviate disease progression. These findings help to elucidate the aetiology and treatment of RA and point us toward the next steps.

Cite this article: Bone Joint Res 2022;11(7):426–438.


Bone & Joint Research
Vol. 10, Issue 11 | Pages 734 - 741
1 Nov 2021
Cheng B Wen Y Yang X Cheng S Liu L Chu X Ye J Liang C Yao Y Jia Y Zhang F

Aims

Despite the interest in the association of gut microbiota with bone health, limited population-based studies of gut microbiota and bone mineral density (BMD) have been made. Our aim is to explore the possible association between gut microbiota and BMD.

Methods

A total of 3,321 independent loci of gut microbiota were used to calculate the individual polygenic risk score (PRS) for 114 gut microbiota-related traits. The individual genotype data were obtained from UK Biobank cohort. Linear regressions were then conducted to evaluate the possible association of gut microbiota with L1-L4 BMD (n = 4,070), total BMD (n = 4,056), and femur total BMD (n = 4,054), respectively. PLINK 2.0 was used to detect the single-nucleotide polymorphism (SNP) × gut microbiota interaction effect on the risks of L1-L4 BMD, total BMD, and femur total BMD, respectively.


Bone & Joint Research
Vol. 10, Issue 10 | Pages 668 - 676
1 Oct 2021
Liu L Li Z Chen S Cui H Li X Dai G Zhong F Hao W Zhang K Liu H

Aims

Acquired heterotopic ossification (HO) is a debilitating disease characterized by abnormal extraskeletal bone formation within soft-tissues after injury. The exact pathogenesis of HO remains unknown. It was reported that BRD4 may contribute to osteoblastic differentiation. The current study aims to determine the role of BRD4 in the pathogenesis of HO and whether it could be a potential target for HO therapy.

Methods

Achilles tendon puncture (ATP) mouse model was performed on ten-week-old male C57BL/6J mice. One week after ATP procedure, the mice were given different treatments (e.g. JQ1, shMancr). Achilles tendon samples were collected five weeks after treatment for RNA-seq and real-time quantitative polymerase chain reaction (RT-qPCR) analysis; the legs were removed for micro-CT imaging and subsequent histology. Human bone marrow mesenchymal stem cells (hBMSCs) were isolated and purified bone marrow collected during surgeries by using density gradient centrifugation. After a series of interventions such as knockdown or overexpressing BRD4, Alizarin red staining, RT-qPCR, and Western Blot (Runx2, alkaline phosphatase (ALP), Osx) were performed on hBMSCs.


Aims

Monocyte-lymphocyte ratio (MLR) or neutrophil-lymphocyte ratio (NLR) are useful for diagnosing periprosthetic joint infection (PJI), but their diagnostic values are unclear for screening fixation-related infection (FRI) in patients for whom conversion total hip arthroplasty (THA) is planned after failed internal fixation for femoral neck fracture.

Methods

We retrospectively included 340 patients who underwent conversion THA after internal fixation for femoral neck fracture from January 2008 to September 2020. Those patients constituted two groups: noninfected patients and patients diagnosed with FRI according to the 2013 International Consensus Meeting Criteria. Receiver operating characteristic (ROC) curves were used to determine maximum sensitivity and specificity of these two preoperative ratios. The diagnostic performance of the two ratios combined with preoperative CRP or ESR was also evaluated.


Bone & Joint Research
Vol. 9, Issue 3 | Pages 130 - 138
1 Mar 2020
Qi X Yu F Wen Y Li P Cheng B Ma M Cheng S Zhang L Liang C Liu L Zhang F

Aims

Osteoarthritis (OA) is the most prevalent joint disease. However, the specific and definitive genetic mechanisms of OA are still unclear.

Methods

Tissue-related transcriptome-wide association studies (TWAS) of hip OA and knee OA were performed utilizing the genome-wide association study (GWAS) data of hip OA and knee OA (including 2,396 hospital-diagnosed hip OA patients versus 9,593 controls, and 4,462 hospital-diagnosed knee OA patients versus 17,885 controls) and gene expression reference to skeletal muscle and blood. The OA-associated genes identified by TWAS were further compared with the differentially expressed genes detected by the messenger RNA (mRNA) expression profiles of hip OA and knee OA. Functional enrichment and annotation analysis of identified genes was performed by the DAVID and FUMAGWAS tools.


Bone & Joint Research
Vol. 7, Issue 5 | Pages 343 - 350
1 May 2018
He A Ning Y Wen Y Cai Y Xu K Cai Y Han J Liu L Du Y Liang X Li P Fan Q Hao J Wang X Guo X Ma T Zhang F

Aim

Osteoarthritis (OA) is caused by complex interactions between genetic and environmental factors. Epigenetic mechanisms control the expression of genes and are likely to regulate the OA transcriptome. We performed integrative genomic analyses to define methylation-gene expression relationships in osteoarthritic cartilage.

Patients and Methods

Genome-wide DNA methylation profiling of articular cartilage from five patients with OA of the knee and five healthy controls was conducted using the Illumina Infinium HumanMethylation450 BeadChip (Illumina, San Diego, California). Other independent genome-wide mRNA expression profiles of articular cartilage from three patients with OA and three healthy controls were obtained from the Gene Expression Omnibus (GEO) database. Integrative pathway enrichment analysis of DNA methylation and mRNA expression profiles was performed using integrated analysis of cross-platform microarray and pathway software. Gene ontology (GO) analysis was conducted using the Database for Annotation, Visualization and Integrated Discovery (DAVID).


Orthopaedic Proceedings
Vol. 94-B, Issue SUPP_XXIV | Pages 13 - 13
1 May 2012
Roposch A Liu L Kulaveerasingham D
Full Access

Purpose of the study

To determine the consistency with which specialist orthopaedic surgeons rate the importance of criteria for the diagnosis of DDH which had previously been generated in a BSCOS and EPOS multi national study.

Methods

163 members of BSCOS received a questionnaire containing 37 criteria that, in a previous study, had been found to be important for the diagnosis of DDH in infants under 8 weeks. A visual analogue scale was used to rate the importance of each criterion in the diagnosis of DDH. We determined the consistency using the intra-class correlation coefficient (values > .80 are anticipated). Analyses were preformed for all members of BSCOS and for geographic regions.


Orthopaedic Proceedings
Vol. 92-B, Issue SUPP_I | Pages 24 - 25
1 Mar 2010
Gooch K Hibbert J Khong H Liu L Dort L Smith D Wasylak T Frank CB William D Johnston C Pearce TJ Zernicke RF
Full Access

Purpose: Elective total hip and knee replacement surgeries are effective procedures for patients suffering from hip and knee disease. The demand for joint replacements is expected to rise as the life expectancy of Canadians increases; thus putting a heavy burden on healthcare. In an effort to reduce the acute hospital length of stay (LOS) the Alberta Orthopaedic Society, with the Alberta Bone and Joint Health Institute, three Alberta health regions (Calgary, Capital and David Thompson) and Alberta Health and Wellness created an evidence based new care continuum for hip and knee replacement. The LOS through the new care continuum compared to the current conventional approach was evaluated. In addition patient characteristics that could potentially predict the LOS were evaluated.

Method: The study design was a randomized, controlled trial. Consenting subjects were randomized to receive care through either the new care continuum (intervention) or the existing “current conventional approach” (control). Acute hospital LOS was calculated as the difference between the date and time the patient was admitted to the date and time the patient was discharged. Data was collected on patient characteristics potentially associated with acute hospital LOS.

Results: Intervention patients demonstrated a significantly shorter acute hospital LOS than the control patients, 4.66 and 5.95 days respectively. Further analysis of the data using a generalized linear model indicated that several patient characteristics were associated with a shorter/longer wait for consultation and surgery. Married patients had a statistically significant shorter LOS than single patients (IRR=0.89, p=0.001). Whereas older patients (IRR=1.01, p=< 0.001), patients with increased comorbidity (IRR= 1.03, p=0.001), and patients with an ASA of ≥ 3 (IRR= 1.22, p=< 0.001) resulted in a significantly longer LOS.

Conclusion: This study indicated that an evidence based healthcare continuum for the delivery of hip and knee replacements was successful in significantly reducing acute care LOS. Reducing the LOS using the new care continuum could potentially help alleviate the strain on limited healthcare resources and the savings could be reinvested to increase the numbers of joint replacement performed. Furthermore, an understanding of patient characteristics that influence acute hospital care LOS should be used to model surgical case mixing to further improve efficiencies.


Orthopaedic Proceedings
Vol. 92-B, Issue SUPP_I | Pages 12 - 12
1 Mar 2010
Gooch K Wasylak T Dort L Smith D Khong H Hibbert J Liu L Frank CB William D Johnston C Pearce TJ Zernicke RF
Full Access

Purpose: Total hip and knee replacements are recognized as effective surgical interventions for severe arthritis. Increasing demand for these services has led to increasing waits for both consultation and surgery. The Alberta Orthopaedic Society, with the Alberta Bone and Joint Health Institute, the Calgary Health Region, the Capital Health Region, the David Thompson Health Region and Alberta Health and Wellness carried out an analysis and an evidence based redesign of the care continuum for hip and knee replacement surgery in an effort to reduce wait times and improve the quality of service for patients.

Method: The study design was a randomized, controlled trial. Consenting subjects were randomized to receive care through either the new care continuum or the existing “current conventional approach”.

Results: During the 12 month study, 1570 patients received surgery. The mean wait for an orthopaedic consultation for intervention patients was 106 days compared to 200 days for the control patients. The mean wait for hip or knee surgery for intervention patients was 157 days compared to 408 for the control patients. Further analysis of the data using a generalized linear model utilizing negative binomial regression indicated that several patient characteristics were associated with a shorter/longer wait times. The less the severity of the disease pre-surgery was associated with a longer wait for a consult (IRR=1.01, p=< 0.001). Longer waits for surgery were statistically associated with less disease severity (IRR=1.004, P=0.019), documented delay (due to non-arthritic medical concerns or patient request for delay, IRR=1.61, p=< 0.001), increased comorbidity (IRR=1.03, p=0.015), and smoking (IRR=1.30, p=0.020).

Conclusion: The results of this study confirm that a redesigned joint replacement new care continuum with a standardized primary care referral process to centralized, specialized joint replacement clinics without established backlogs can help reduce wait times in Canada.