Introduction

Gait analysis systems have enjoyed increasing usage and have been validated to provide highly accurate assessments for range of motion. Size, cost, need for marker placement and need for complex data processing have remained limiting factors in uptake outside of what remains predominantly large research institutions. Progress and advances in deep neural networks, trained on millions of clinically labelled datasets, have allowed the development of a computer vision system which enables assessment using a handheld smartphone with no markers and accurate range of motion for knee during flexion and extension. This allows clinicians and therapists to objectively track progress without the need for complex and expensive equipment or time-consuming analysis, which was concluded to be lacking during a recent systematic review of existing applications.

Introduction: As candidates for arthroplasty become younger and life expectancy increases the required working life of a total hip arthroplasty continues to rise. Hip resurfacing offers potential further advantages in young patients as minimal bone resection makes for easier revision, and the design allows for an increased range of movement. The Birmingham Hip Resurfacing (BHR) is the first of the second generation hip resurfacings.

Reports are beginning to emerge of unexplained failure, pseudotumour formation, individual cases of metallosis. Joint registry data also demonstrates an unexplained high early failure rate for all designs of hip resurfacing. This paper examines the rate and mode of early failures of the BHR in a multi-centre, multi-surgeon series.

Methods: All patients undergoing BHRs in our two centres were recruited prospectively into our arthroplasty follow up programme. Patients have been followed up radiographically and with clinical scores.

Results: Mean radiographic and clinical follow up was to 43 months (range 6 – 90 months). Of the 463 BHRs two have died and three are lost to follow up. Thirteen arthroplasties (2.8%) have been revised. Eight for pain, three for fracture, two for dislocation and one for sepsis. Of these nine were found to have macroscopic and histological evidence of metalloisis. Survival analysis at 5 years is 95.8% (CI 94.1 – 96.8%) for revisions and 96.9% (CI 95.5 – 98.3%) for metallosis.

Discussion: Histopathological examination demonstrated a range of inflammatory changes including necrosis, inflammation, ALVAL and metal containing macrophages. Not all features were associated with each patient and it is likely that these features form part of the spectrum of metal wear debris disease.

The likely rate of metallosis is 3.1% at five years. Risk factors for metallosis in this series are female sex, small femoral component, high abduction angle and obesity. We not advocate use of the BHR in patients with these risk factors.

Introduction: It is well known that the fate of biomaterials is determined by the distribution of proteins attached to the surface from the initial contact with blood or serum. This profile determines wether a material is inert, creates a foreign body response or is bioactive. Bioinert materials, such as polyethylene completely denature surface proteins, whilst materials inducing inflammatory responses are predisposed to complement protein attachment. Bioactive materials such autologous tissue grafts adsorb, but do not denature serum proteins such as fibronectin and Von Willebrand’s factor. This does not interfere with the healing cascade. This aim of this study is to prepare a synthetic bone graft substitute that activates the body’s autologous healing cascade by activating platelets, without activating a complement response through the controlled adsorption of serum proteins.

Methods: Polymers composed of varied concentration of acrylic acid (AA) and comonomers (methyl, ethyl and butyl methacrylates (MMA, EMA, BMA)) were prepared in glass vials by free radical polymerisation. Fresh blood was collected from a healthy donor and pipetted immediately into each chamber. Glass was used as a control. The chambers were incubated at 37o C for 2 hours. The surface morphology was examined using Scanning Electron Microscopy (SEM). Concentration of complement protein C5a and prothrombin fragments 1 and 2 were determined using commercial ELISA kits. Foreign body reaction (FBR) initiated by the biomaterial was estimated by counting leukocytes on clot sections using immunofluorescence.

Results: Extent of coagulation was correlated with plasma concentrations of Prothrombin fragments 1 and 2. These measurements show blood incubated with various polymers composed of different comonomers all promoted the formation of blood clots. It was found that the leukocyte population towards the interface of clot and polymer (AA:MMA) decreased with increasing surface acid concentration (65%AA:MMA 30 leukocytes/0.25mm2, glass 70 leukocytes/0.25mm2 (p< 0.05)). FBR is induced by the activation of complement system. The percentage of C5a concentration detected in blood incubated with various polymers composed of different comonomers relative to normal serum level of C5a (35ng/mL). No significant elevations of C5a were measured from polymer 65% AA:MMA and 65% AA:EMA. Glass induced vigorous complement response as expected. The synergistic combination of surface acid concentration and comonomers had a significant effect on extent of FBR. Increased acid concentration resulted in decreased C5a level with MMA and ET but increased level with BMA.

Discussion: The functional groups exposed on the surface of a material influence whether leukocyte or platelet activation is responsible for the subsequent physiological response. By modifying the combinations of surface acid concentrations and comonomers, we show that a biomaterial with an appropriate surface chemistry promotes the platelet plug formation and coagulation but down regulated foreign body reaction. This study shows that that a biomaterial with the appropriate surface chemistry to evoke the same coagulation response as damaged tissue, mediated through platelet activation and intrinsic and extrinsic coagulation, initiates the initial pathways of the bone healing cascade. This material is a realistic candidate for biomaterial induced bone regeneration.

Introduction: Acute neurological damage from spinal cord injuries is believed to be localised, however it initiates a cascade of secondary events which usually leads to extensive and permanent neurological deficit. The secondary damage begins with the disruption of the blood-spinal cord barrier which unleashes a protracted inflammatory response. This prolonged inflammatory response is the catalyst for the secondary neurodegeneration and limited repair response that occurs in the chronic phase of a spinal cord injury. In this study it was proposed that the acute delivery of the angiogenic growth factors vascular endothelial growth factor (VEGF) and platelet derived growth factor (PDGF) would mediate inflammation and restore the blood spinal cord barrier. This would minimise the formation of glial scar and reduce the extent of secondary degeneration caudal and cranial to the lesion site.

Methods: Adult male Wistar rats (400g) were anesthetised. Complete laminectomies were performed at T10 and the animals were subjected to T10 hemisection. Animals were randomised to a treatment group (Lesion Control (LC), Gel Control (GC) and Angiogenic Gel (AG)) after the spinal cord was cut. Each treatment group had 6 animals sacrificed 3 months post injury. Sections were stained with antibodies to neurofilament 200, glial fibrillary acidic protein, smooth muscle actin (SMA), and fluorescent secondary antibodies and mounted with DAPI. The lesion size was measured from horizontal histological sections of the midline from 5 animals in each group using Axiovision version 4.6.1.0 (Carl Zeiss Imaging Solutions, Germany).

Results: The mean lesion size for the lesion control group was 2.09mm2, 1.97mm2 for the gel control group and 0.45mm2 for the active gel group. A t-test was used to confirm that the differences between the active gel and the two control groups were statistically significant (AG vs LC p= 0.021 AG vs GC p= 0.026). Histology showed a marked improvement of the morphology of the astrocytes in the treatment group over the control groups indicating that the treatment affected the population of reactive astrocytes. SMA staining showed an increased level of revascularisation in the treated lesions.

Discussion: Spinal cords do not heal because of prolonged inflammation which leads to secondary necrotic events, scar formation and the inhibition of regeneration. In this study we present a method for regulating the post lesion inflammatory signals, significantly reducing post-lesion scar formation. We propose the delivery of VEGF/PDGF significantly increases the permeability of the blood spinal cord barrier to neutrophils and macrophages and promotes angiogenesis observed in the lesion site. This may have two major effects on the progression of the spinal cord injury. Firstly, by increasing the initial influx of inflammatory cells it enables the faster removal of damaged tissue and phagocytosis of apoptotic cells thereby restoring the balance in favour of regulated inflammation and results in a finite and reduced inflammation time. Secondly, combination of VEGF and PDGF provides a robust angiogenic response and reduces ischemia, the population of reactive astrocytes and the capacity to form glial scars. These growth factors appear to moderate the secondary degenerative changes that result from the prolonged inflammation and thus promote the inherent capacity for regeneration.

60 out of total series of 643 metal-on-metal hip replacements, carried out over the last nine years, have so far required revision, 13 for peri-prosthetic fracture and 47 for extensive, symptomatic, peri-articular soft-tissue changes.

Dramatic corrosion of generally solidly fixed, cemented stems has been observed and is believed to have resulted in the release of high levels of cobalt chrome ions from the stem surface. The contribution of the metal-to-metal articulation is, as yet, unclear.

Not including the fracture cases, plain films have demonstrated little or no abnormality to account for patients’ progressive symptoms. MRI scanning, on the other hand, utilising a technique designed to minimise implant artefact, has correlated very closely with findings at the time of revision surgery.

The histological changes, typified by extensive lymphocytic infiltration and a severe vasculitis leading to, in some cases, extensive tissue necrosis are demonstrated and discussed.

The failure of any of the existing protective mechanisms or regulatory restrictions to identify and limit the exposure of large numbers of patients to unsatisfactory implants has again been demonstrated.

Introduction Over recent years the techniques of femoral and acetabular impaction allografting with fresh frozen morsellised bone have become incressingly popular for revision total hip arthoplasty with osseous defects. In many centres lack of availability or legislation has required surgeons to explore alternatives to fresh frozen bone that may have different structural and biological properties. In this study we compare in vitro the load carrying capacity of irradiated morsellised bone against a control non-irradiated sample.

Methods Fresh frozen heads were divided in halves with one half irradiated at 25 kGy and the control half left non-irradiated. A custom-built pneumatic loading apparatus applied a force of 1200N at a cycle rate of 1Hz for a total of 1500 cylcles. This loading cycle was chosen to simulate the loads normally experienced by the human femur during walking gait. The reduction in height (subsidence) of each test specimen was measured and statistical analysis performed.

Results Results from each treatment group displayed similar patterns of subsidence, with an initial rapid rate of subsidence occurring up to 50 to 100 load cycles, followed by a more gradual, slower rate as the tests progressed. The results for each treatment (mean ± standard deviation) were −3.59 ± 0.91 mm and −2.98 ± 0.812 mm for the irradiated and non-irradiated groups, respectively (P+0.049). The irradiated specimens demonstrated an increased amount of subsidence compared to the non-irradiated specimens.

Conclusions This study has shown that gamma irradiation of morsellised bone allograft material decreases its load-carrying capacity, as expressed by an increase in subsidence due to an applied cyclic load. The ability for morsellised bone allograft material to bear applied loads in vivo is an important biomechanical parameter and one indicator of a successful clinical outcome. The clinical implications of this result are important when considering the most appropriate methods of treating human bone allograft material.

In relation to the conduct of this study, one or more of the authors is in receipt of a research grant from a commercial source.

INTRODUCTION: Structural changes to the intervertebral disc (IVD) in the form of anular lesions are a feature of IVD degeneration. Degeneration has been related to changes in the mechanical function of the IVD. This study determined the mechanical effect of individual concentric tears, radial tears and rim lesions of the anulus in an in vitro experiment.

METHODS: The lumbar spines from five sheep were taken post mortem and divided into three motion segments. The disc body units were tested on a robotic testing facility, using position control, in flexion/extension, lateral bending and axial rotation. Concentric tears, radial tears and rim lesions were experimentally introduced and the motions repeated after the introduction of each lesion. The mechanical response after the lesion creation was compared to the undamaged response to assess the mechanical effect of each lesion.

RESULTS: It was found that an anterior rim lesion reduced the peak moment resisted by the disc in extension, lateral bending and axial rotation. Concentric tears and radial tears did not affect the peak moment resisted, however, radial tears reduced the hysteresis of response in flexion/extension and lateral bending. The neutral zone was not affected by the presence of IVD lesions.

DISCUSSION: These results show that rim lesions reduce the disc’s ability to resist motion. Radial tears change the hysteresis of response indicating an altered stress distribution in the disc. These changes may lead to overloading of the spinal ligaments, muscles and zygapophysial joints, possibly damaging these structures. This suggests a mechanism for a cycle of degeneration that is instigated by small changes in the mechanical integrity of the IVD.

Introduction: the neutral zone is defined as a region of no or little resistance to motion in the middle of an intervertebral joint’s range of movement. Previous studies have used quasistatic loading regimes that do not model physiological activity¹. The aim of the present study was to assess experimentally the existence of the neutral zone of intervertebral joints during spinal motion in flexion/extension, lateral bending and axial rotation during physiological movements simulated using a robotic testing facility. Sheep intervertebral joints were used as they have been shown to exhibit similar mechanical behaviour to human joints².

Methods: five spines from mature sheep were used. Three specimens were tested from each spine to simulate human l1/2, l3/4 and l4/5 intervertebral joints. The robotic facility enabled the testing regime to be defined for each individual joint based on its geometry. The joints were tested by cycling through the full range of physiological movement in flexion/extension, lateral bending and axial rotation.

Results: a neutral zone was found to exist during dynamic movements only in flexion/extension. The results were equivocal for lateral bending and suggested that a neutral zone does not exist in axial rotation. The zygapophysial joints were shown to be significant in determining the mechanics of the intervertebral joints as their removal increased the neutral zone in all cases. A criterion for defining the size of the neutral zone was proposed.

Conclusions: a neutral zone exists in flexion/extension during dynamic movements of intervertebral joints. This has important implications for the muscular control of the spine consisting of several intrinsically lax joints stacked on one another.